Cloned (Comment) | Organism |
---|---|
gene dohh, sequence comparison, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Plasmodium vivax |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
zileuton | - |
Plasmodium vivax |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39010 | - |
x * 39010, recombinant His-tagged enzyme, SDS-PAGE | Plasmodium vivax |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[eIF5A]-deoxyhypusine + AH2 + O2 | Plasmodium vivax | - |
[eIF5A]-hypusine + A + H2O | - |
? | |
[eIF5A]-deoxyhypusine + AH2 + O2 | Plasmodium vivax Salvador I | - |
[eIF5A]-hypusine + A + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium vivax | A5JZ19 | gene dohh, substrain PEXT-1 | - |
Plasmodium vivax Salvador I | A5JZ19 | gene dohh, substrain PEXT-1 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affiniity chromatography | Plasmodium vivax |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[eIF5A]-deoxyhypusine + AH2 + O2 | - |
Plasmodium vivax | [eIF5A]-hypusine + A + H2O | - |
? | |
[eIF5A]-deoxyhypusine + AH2 + O2 | - |
Plasmodium vivax | [eIF5A]-hypusine + A + H2O | product analysis by GC/MS and gel filtration | ? | |
[eIF5A]-deoxyhypusine + AH2 + O2 | - |
Plasmodium vivax Salvador I | [eIF5A]-hypusine + A + H2O | - |
? | |
[eIF5A]-deoxyhypusine + AH2 + O2 | - |
Plasmodium vivax Salvador I | [eIF5A]-hypusine + A + H2O | product analysis by GC/MS and gel filtration | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39010, recombinant His-tagged enzyme, SDS-PAGE | Plasmodium vivax |
Synonyms | Comment | Organism |
---|---|---|
deoxyhypusine hydroxylase | - |
Plasmodium vivax |
DOHH | - |
Plasmodium vivax |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Plasmodium vivax |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Plasmodium vivax |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0000125 | - |
recombinant His-tagged enzyme, pH 7.5, 37°C | Plasmodium vivax | zileuton |
General Information | Comment | Organism |
---|---|---|
physiological function | deoxyhypusine hydroxylase completes hypusine biosynthesis in eukaryotic initiation factor (eIF-5A) which is the only cellular protein known to contain the unusual amino acid hypusine. Modified EIF-5A is important for proliferation of the malaria parasite | Plasmodium vivax |