Literature summary for 1.14.99.29 extracted from

Frommholz, D.; Kusch, P.; Blavid, R.; Scheer, H.; Tu, J.M.; Marcus, K.; Zhao, K.H.; Atemnkeng, V.; Marciniak, J.; Kaiser, A.E.
Completing the hypusine pathway in Plasmodium (2009), FEBS J., 276, 5881-5891.

Search for more literature for 1.14.99.29

Cloned(Commentary)

Cloned (Comment)	Organism
into the pST-Acceptor vector for sequencing, into the vectors pET-15b and pET-28a for expression in Escherichia coli BL21DE3 cells	Plasmodium falciparum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
42000	-	histidine-tagged recombinant DOHH, determined by SDS-PAGE	Plasmodium falciparum
46450	-	theoretical	Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Plasmodium falciparum	DOHH activity completes hypusine biosynthesis via hydroxylation and thereby completes eukaryotic translation initiation factor eIF-5A formation	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Plasmodium falciparum	C9QNK6	-	-

Purification (Commentary)

Purification (Comment)	Organism
performed by nickel-chelate affinity chromatography under native conditions	Plasmodium falciparum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	DOHH activity completes hypusine biosynthesis via hydroxylation and thereby completes eukaryotic translation initiation factor eIF-5A formation	Plasmodium falciparum	?	-	?

Synonyms

Synonyms	Comment	Organism
deoxyhypusine hydroxylase	-	Plasmodium falciparum
DOHH	-	Plasmodium falciparum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	activity assay	Plasmodium falciparum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	activity assay	Plasmodium falciparum

pI Value

Organism	Comment	pI Value Maximum	pI Value
Plasmodium falciparum	theoretical	-	4.96

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Release 2023.1 (January 2023)