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Literature summary for 1.14.99.29 extracted from

  • Huang, J.K.; Cui, Y.; Chen, C.H.; Clampitt, D.; Lin, C.T.; Wen, L.
    Molecular cloning and functional expression of bovine deoxyhypusine hydroxylase cDNA and homologs (2007), Protein Expr. Purif., 54, 126-133.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, different clones, sequence comparisons, expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain JM109 Bos taurus

Protein Variants

Protein Variants Comment Organism
E57G site-directed mutagenesis, the substitution located at one of the four conserved His-Glu pairs, the potential metal coordination sites, results in severe reduction of deoxyhypusine hydroxylase activity compared to the wild-type enzyme Bos taurus
additional information a deletion at the HEAT-repeats 1-3, potential metal coordination sites, results in complete losses of deoxyhypusine hydroxylase activity, the different clones encoding the enzyme show differing activity, overview Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
x * 33000, about, sequence calculation Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
eIF5A-Nepsilon-(4-aminobutyl)lysine + AH2 + O2 Bos taurus the enzyme catalyzes the maturation of eukaryotic initiation factor 5A, synthesis of hypusine is essential for the function of eIF5A in eukaryotic cell proliferation and survival eIF5A-Nepsilon-(4-amino-2-hydroxybutyl)lysine + A + H2O
-
?
additional information Bos taurus eIF5A occurs in tumor cells, and it also acts as a cofactor of the Rev transactivator protein in HIV-1-infected cells ?
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus Q0VC53
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli strain JM109 by glutathione affinity chromatography, the GST-tag is cleaved off Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
eIF5A-Nepsilon-(4-aminobutyl)lysine + AH2 + O2 the enzyme catalyzes the maturation of eukaryotic initiation factor 5A, synthesis of hypusine is essential for the function of eIF5A in eukaryotic cell proliferation and survival Bos taurus eIF5A-Nepsilon-(4-amino-2-hydroxybutyl)lysine + A + H2O
-
?
eIF5A-Nepsilon-(4-aminobutyl)lysine + AH2 + O2 eIF5A is the eukaryotic translation initiation factor 5A Bos taurus eIF5A-Nepsilon-(4-amino-2-hydroxybutyl)lysine + A + H2O
-
?
additional information eIF5A occurs in tumor cells, and it also acts as a cofactor of the Rev transactivator protein in HIV-1-infected cells Bos taurus ?
-
?

Subunits

Subunits Comment Organism
? x * 33000, about, sequence calculation Bos taurus

Synonyms

Synonyms Comment Organism
deoxyhypusine hydroxylase
-
Bos taurus

pI Value

Organism Comment pI Value Maximum pI Value
Bos taurus about, sequence calculation
-
4.9