Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, different clones, sequence comparisons, expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain JM109 | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
E57G | site-directed mutagenesis, the substitution located at one of the four conserved His-Glu pairs, the potential metal coordination sites, results in severe reduction of deoxyhypusine hydroxylase activity compared to the wild-type enzyme | Bos taurus |
additional information | a deletion at the HEAT-repeats 1-3, potential metal coordination sites, results in complete losses of deoxyhypusine hydroxylase activity, the different clones encoding the enzyme show differing activity, overview | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
x * 33000, about, sequence calculation | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
eIF5A-Nepsilon-(4-aminobutyl)lysine + AH2 + O2 | Bos taurus | the enzyme catalyzes the maturation of eukaryotic initiation factor 5A, synthesis of hypusine is essential for the function of eIF5A in eukaryotic cell proliferation and survival | eIF5A-Nepsilon-(4-amino-2-hydroxybutyl)lysine + A + H2O | - |
? | |
additional information | Bos taurus | eIF5A occurs in tumor cells, and it also acts as a cofactor of the Rev transactivator protein in HIV-1-infected cells | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | Q0VC53 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli strain JM109 by glutathione affinity chromatography, the GST-tag is cleaved off | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
eIF5A-Nepsilon-(4-aminobutyl)lysine + AH2 + O2 | the enzyme catalyzes the maturation of eukaryotic initiation factor 5A, synthesis of hypusine is essential for the function of eIF5A in eukaryotic cell proliferation and survival | Bos taurus | eIF5A-Nepsilon-(4-amino-2-hydroxybutyl)lysine + A + H2O | - |
? | |
eIF5A-Nepsilon-(4-aminobutyl)lysine + AH2 + O2 | eIF5A is the eukaryotic translation initiation factor 5A | Bos taurus | eIF5A-Nepsilon-(4-amino-2-hydroxybutyl)lysine + A + H2O | - |
? | |
additional information | eIF5A occurs in tumor cells, and it also acts as a cofactor of the Rev transactivator protein in HIV-1-infected cells | Bos taurus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33000, about, sequence calculation | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
deoxyhypusine hydroxylase | - |
Bos taurus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bos taurus | about, sequence calculation | - |
4.9 |