Literature summary for 1.14.99.29 extracted from

Csonga, R.; Ettmayer, P.; Auer, M.; Eckerskorn, C.; Eder, J.; Klier, H.
Evaluation of the metal ion requirement of the human deoxyhypusine hydroxylase from HeLa cells using a novel enzyme assay (1996), FEBS Lett., 380, 209-214.

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Inhibitors

Inhibitors	Comment	Organism	Structure
2,2'-dipyridyl	IC50 0.029 mM	Homo sapiens
4,6-diphenyl-1-hydroxy-pyridine-2-one	IC50 0.0007 mM	Homo sapiens
ciclopirox	IC50 0.0006 mM	Homo sapiens
Co(C2H3O2)2	above 0.01 mM	Homo sapiens
CuCl2	above 0.01 mM	Homo sapiens
EDTA	IC50 0.0003 mM	Homo sapiens
metipirox	IC50 0.0028 mM	Homo sapiens
mimosine	IC50 0.0033 mM	Homo sapiens
Mn(C2H3O2)2	above 0.001 mM	Homo sapiens
additional information	partial reconstitution of chelator inhibited activity with CuCl2 or Zn(C2H3O2)2	Homo sapiens
Ni(C2H3O2)2	above 0.001 mM	Homo sapiens
Zn(C2H3O2)2	above 0.01 mM	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	1.5-fold activation at 0.001 mM	Homo sapiens
Fe3+	1.5-fold activation at 0.001 mM	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
HeLa-S3 cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N'-(4-aminobutyl)lysine + electron donor + O2	i.e. deoxyhypusine	Homo sapiens	N'-(4-amino-2-hydroxybutyl)lysine + oxidized electron donor + H2O	i.e. hypusine	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay in 50 mM sodium phosphate buffer	Homo sapiens

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.0003	-	IC50 0.0003 mM	Homo sapiens	EDTA
0.0006	-	IC50 0.0006 mM	Homo sapiens	ciclopirox
0.0007	-	IC50 0.0007 mM	Homo sapiens	4,6-diphenyl-1-hydroxy-pyridine-2-one
0.0028	-	IC50 0.0028 mM	Homo sapiens	metipirox
0.0033	-	IC50 0.0033 mM	Homo sapiens	mimosine
0.029	-	IC50 0.029 mM	Homo sapiens	2,2'-dipyridyl

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Release 2023.1 (January 2023)