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Literature summary for 1.14.99.24 extracted from

  • Strijewski A.
    The steroid-9alpha-hydroxylation system from Nocardia species (1982), Eur. J. Biochem., 128, 125-135.
    View publication on PubMed
Search for more literature for 1.14.99.24

Application

Application Comment Organism
synthesis enzyme occurs in many bacterial genera used in industrial processes Corynebacterium sp.
synthesis enzyme occurs in many bacterial genera used in industrial processes Arthrobacter sp.
synthesis enzyme occurs in many bacterial genera used in industrial processes Mycobacterium sp.
synthesis enzyme occurs in many bacterial genera used in industrial processes Nocardia sp.

Inhibitors

Inhibitors Comment Organism Structure
2,2'-bipyridine
-
 Corynebacterium sp.
2,2'-bipyridine 52% inhibition Nocardia sp.
Cd(CH3COO)2 80% inhibition Nocardia sp.
CuSO4 100% inhibition Nocardia sp.
Hg(CH3COO)2 33% inhibition Nocardia sp.
o-phenanthroline
-
 Corynebacterium sp.
o-phenanthroline
-
 Mycobacterium sp.
o-phenanthroline 72% inhibition Nocardia sp.
potassium cyanide 60% inhibition Nocardia sp.
Sodium azide 100% inhibition Nocardia sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ iron-sulfur protein Nocardia sp.
Mg2+ higher hydroxylation obtained by increasing magnesium ion concentration with a maximum of stimulation at 20 mM or more Nocardia sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
 hydroxylase system composed of 3 proteins, protein III, comparative gel filtration Nocardia sp.
214000
-
 hydroxylase system composed of 3 proteins, protein II, comparative gel filtration Nocardia sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4,9(11)-pregnadiene-3,20-dione + NADH + O2 Corynebacterium sp.
-
 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
 ?
4,9(11)-pregnadiene-3,20-dione + NADH + O2 Arthrobacter sp.
-
 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
 ?
4,9(11)-pregnadiene-3,20-dione + NADH + O2 Mycobacterium sp.
-
 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
 ?
4,9(11)-pregnadiene-3,20-dione + NADH + O2 Nocardia sp. key-enzyme in the steroid-ring-B-splitting pathway 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
 ?
4,9(11)-pregnadiene-3,20-dione + NADH + O2 Nocardia sp. M117 key-enzyme in the steroid-ring-B-splitting pathway 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
-
-
Corynebacterium sp.
-
-
-
Mycobacterium sp.
-
-
-
Nocardia sp.
-
-
-
Nocardia sp. M117
-
-
-

Oxidation Stability

Oxidation Stability Organism
protein II and protein III are oxygen-labile Nocardia sp.

Purification (Commentary)

Purification (Comment) Organism
partially Nocardia sp.

Storage Stability

Storage Stability Organism
-100°C, enzyme activity in clear supernatant frozen in liquid nitrogen is stable for more than 1 year Nocardia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4,9(11)-pregnadiene-3,20-dione + NADH + O2
-
 Corynebacterium sp. 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
 ?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
-
 Arthrobacter sp. 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
 ?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
-
 Mycobacterium sp. 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
 ?
4,9(11)-pregnadiene-3,20-dione + NADH + O2 key-enzyme in the steroid-ring-B-splitting pathway Nocardia sp. 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
 ?
4,9(11)-pregnadiene-3,20-dione + NADH + O2 key-enzyme in the steroid-ring-B-splitting pathway Nocardia sp. M117 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
 ?

Subunits

Subunits Comment Organism
More enzyme system involves a flavoprotein reductase and 2 iron-sulfur proteins Nocardia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Nocardia sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.5 10
-
 Nocardia sp.

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein Nocardia sp.
NADH
-
 Mycobacterium sp.
NADH
-
 Nocardia sp.
NADPH conversion rate higher with NADH Nocardia sp.