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Literature summary for 1.14.99.22 extracted from

  • Horike, N.; Takemori, H.; Nonaka, Y.; Sonobe, H.; Okamoto, M.
    Molecular cloning of NADPH-cytochrome P-450 oxidoreductase from silkworm eggs (2000), Eur. J. Biochem., 267, 6914-6920.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cDNA isolated from non-diapause eggs, expression in Escherichia coli BL21 as GST-fusion protein Bombyx mori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
77700
-
? * 77700, sequence determination Bombyx mori
103000
-
? * 103000, SDS-PAGE, recombinant GST-fusion protein Bombyx mori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ecdysone + NADPH + O2 Bombyx mori
-
20-hydroxyecdysone + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Bombyx mori
-
silkworm
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-fusion protein Bombyx mori

Source Tissue

Source Tissue Comment Organism Textmining
additional information immunological analysis of enzyme content in diapause and non-diapause egg microsomes Bombyx mori
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
27
-
recombinant GST-fusion protein, purified Bombyx mori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-ecdysone + NADPH + O2
-
Bombyx mori 20-hydroxyecdysone + NADP+ + H2O
-
?
ecdysone + NADPH + O2
-
Bombyx mori 20-hydroxyecdysone + NADP+ + H2O
-
?
additional information recombinant GST-fusion protein, expressed in E. coli, is also able to transfer electrons from NADPH to a microsomal steroid hydroxylase p-450 and support steroidogenesis Bombyx mori ?
-
?

Subunits

Subunits Comment Organism
? ? * 77700, sequence determination Bombyx mori
? ? * 103000, SDS-PAGE, recombinant GST-fusion protein Bombyx mori

Cofactor

Cofactor Comment Organism Structure
cytochrome P450 essential Bombyx mori
NADPH
-
Bombyx mori