Cloned (Comment) | Organism |
---|---|
cDNA isolated from non-diapause eggs, expression in Escherichia coli BL21 as GST-fusion protein | Bombyx mori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
77700 | - |
? * 77700, sequence determination | Bombyx mori |
103000 | - |
? * 103000, SDS-PAGE, recombinant GST-fusion protein | Bombyx mori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ecdysone + NADPH + O2 | Bombyx mori | - |
20-hydroxyecdysone + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bombyx mori | - |
silkworm | - |
Purification (Comment) | Organism |
---|---|
recombinant GST-fusion protein | Bombyx mori |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | immunological analysis of enzyme content in diapause and non-diapause egg microsomes | Bombyx mori | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
27 | - |
recombinant GST-fusion protein, purified | Bombyx mori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-ecdysone + NADPH + O2 | - |
Bombyx mori | 20-hydroxyecdysone + NADP+ + H2O | - |
? | |
ecdysone + NADPH + O2 | - |
Bombyx mori | 20-hydroxyecdysone + NADP+ + H2O | - |
? | |
additional information | recombinant GST-fusion protein, expressed in E. coli, is also able to transfer electrons from NADPH to a microsomal steroid hydroxylase p-450 and support steroidogenesis | Bombyx mori | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | ? * 77700, sequence determination | Bombyx mori |
? | ? * 103000, SDS-PAGE, recombinant GST-fusion protein | Bombyx mori |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | essential | Bombyx mori | |
NADPH | - |
Bombyx mori |