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Literature summary for 1.14.99.2 extracted from
- Studies on the metabolism of kynurenic acid (1963), J. Biol. Chem., 238, 283-293.
General Stability
| General Stability | Organism |
|---|---|
| 2-mercaptoethanol stabilizes activity | Pseudomonas fluorescens |
| cysteine stabilizes activity | Pseudomonas fluorescens |
| GSH stabilizes activity | Pseudomonas fluorescens |
| precipitation of supernatant with ammonium sulfate in the absence of cysteine, complete loss of activity | Pseudomonas fluorescens |
| thioglycolate stabilizes activity | Pseudomonas fluorescens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Pseudomonas fluorescens |  |
| Cu2+ | - |
Pseudomonas fluorescens | |
| Hg2+ | - |
Pseudomonas fluorescens | |
| iodoacetate | inhibition reversed by addition of GSH or cysteine | Pseudomonas fluorescens | |
| Kynurenate | competitive inhibition at 0.2 mM | Pseudomonas fluorescens | |
| p-chloromercuribenzoate | inhibition reversed by addition of GSH or cysteine | Pseudomonas fluorescens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
Kynurenate | - |
Pseudomonas fluorescens | |
| 0.04 | - |
NADH | - |
Pseudomonas fluorescens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| kynurenate + NADH + O2 | Pseudomonas fluorescens | - |
7,8-dihydro-7,8-dihydroxykynurenate + NAD+ | - |
? | |
| kynurenate + NADPH + O2 | Pseudomonas fluorescens | - |
7,8-dihydro-7,8-dihydroxykynurenate + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
ATCC 11299b | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Pseudomonas fluorescens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -10°C, ammonium sulfate fractions, loss of activity after a few days | Pseudomonas fluorescens |
| -10°C, supernatant after high speed centrifugation of crude extract, several months, no loss in activity | Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| kynurenate + NADH + O2 | - |
Pseudomonas fluorescens | 7,8-dihydro-7,8-dihydroxykynurenate + NAD+ | - |
? | |
| kynurenate + NADPH + O2 | - |
Pseudomonas fluorescens | 7,8-dihydro-7,8-dihydroxykynurenate + NADP+ | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | - |
assay at | Pseudomonas fluorescens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
5 min, complete inactivation | Pseudomonas fluorescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Pseudomonas fluorescens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
stable in presence 10 mM cysteine | Pseudomonas fluorescens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Pseudomonas fluorescens | |
| NADPH | - |
Pseudomonas fluorescens | |
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Release 2023.1 (January 2023)
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