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Literature summary for 1.14.99.15 extracted from
- Substrate and solvent isotope effects on the fate of the active oxygen species in substrate-modulated reactions of putidamonooxin (2000), Eur. J. Biochem., 267, 5926-5934.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0062 | - |
4-Methylbenzoate | reaction in D2O | Pseudomonas putida |  |
| 0.009 | - |
4-Methylbenzoate | reaction in H2O | Pseudomonas putida | |
| 0.0144 | - |
4-trifluoromethylbenzoate | reaction in D2O | Pseudomonas putida | |
| 0.024 | - |
4-trifluoromethylbenzoate | reaction in H2O | Pseudomonas putida | |
| 0.0367 | - |
benzoate | reaction in H2O | Pseudomonas putida | |
| 0.055 | - |
benzoate | reaction in D2O | Pseudomonas putida | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methoxybenzoate + NADH + O2 | Pseudomonas putida | - |
4-hydroxybenzoate + NAD+ + H2O + formaldehyde | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 194.5 | - |
3-phenyl-4-[2H3]-methoxybenzoate, product formation, reaction in H2O | Pseudomonas putida |
| 207.8 | - |
4-trifluoromethylbenzoate, oxygen uptake, reaction in D2O | Pseudomonas putida |
| 239.8 | - |
3-phenyl-4-[2H3]-methoxybenzoate, product formation, reaction in D2O | Pseudomonas putida |
| 265.6 | - |
benzoate, oxygen uptake, reaction in D2O | Pseudomonas putida |
| 295.1 | - |
benzoate, oxygen uptake, reaction in H2O | Pseudomonas putida |
| 303.9 | - |
3-phenyl-4-[1H3]-methoxybenzoate, product formation, reaction in H2O | Pseudomonas putida |
| 305.2 | - |
4-methylbenzoate, NADH oxidation, reaction in H2O | Pseudomonas putida |
| 310.1 | - |
3-phenyl-4-[2H3]-methoxybenzoate, oxygen uptake, reaction in D2O | Pseudomonas putida |
| 310.1 | - |
3-phenyl-4-[2H3]-methoxybenzoate, oxygen uptake, reaction in H2O | Pseudomonas putida |
| 315.5 | - |
4-trifluoromethylbenzoate, NADH oxidation, reaction in D2O | Pseudomonas putida |
| 317.2 | - |
4-methylbenzoate, NADH oxidation, reaction in D2O | Pseudomonas putida |
| 317.4 | - |
3-phenyl-4-[1H3]-methoxybenzoate, product formation, reaction in D2O | Pseudomonas putida |
| 326.7 | - |
3-phenyl-4-[1H3]-methoxybenzoate, oxygen uptake, reaction in H2O | Pseudomonas putida |
| 329.2 | - |
3-phenyl-4-[1H3]-methoxybenzoate, oxygen uptake, reaction in D2O | Pseudomonas putida |
| 339.1 | - |
benzoate, NADH oxidation, reaction in D2O | Pseudomonas putida |
| 340.2 | - |
3-phenyl-4-[2H3]-methoxybenzoate, NADH oxidation, reaction in H2O | Pseudomonas putida |
| 345 | - |
4-trifluoromethylbenzoate, oxygen uptake, reaction in H2O | Pseudomonas putida |
| 345.6 | - |
benzoate, NADH oxidation, reaction in H2O | Pseudomonas putida |
| 353.5 | - |
3-phenyl-4-[2H3]-methoxybenzoate, NADH oxidation, reaction in D2O | Pseudomonas putida |
| 360.4 | - |
3-phenyl-4-[1H3]-methoxybenzoate, NADH oxidation, reaction in H2O | Pseudomonas putida |
| 367 | - |
4-methylbenzoate, oxygen uptake, reaction in H2O | Pseudomonas putida |
| 368 | - |
4-methylbenzoate, oxygen uptake, reaction in D2O | Pseudomonas putida |
| 380.2 | - |
3-phenyl-4-[1H3]-methoxybenzoate, NADH oxidation, reaction in D2O | Pseudomonas putida |
| 391.5 | - |
4-methoxybenzoate, oxygen uptake, reaction in D2O | Pseudomonas putida |
| 394.1 | - |
4-methoxybenzoate, oxygen uptake, reaction in H2O | Pseudomonas putida |
| 402.6 | - |
3-nitro-4-methoxybenzoate, NADH oxidation, reaction in D2O | Pseudomonas putida |
| 409.7 | - |
4-trifluoromethylbenzoate, NADH oxidation, reaction in H2O | Pseudomonas putida |
| 422.1 | - |
3-nitro-4-methoxybenzoate, NADH oxidation, reaction in H2O | Pseudomonas putida |
| 432.4 | - |
3-nitro-4-methoxybenzoate, oxygen uptake, reaction in H2O | Pseudomonas putida |
| 432.7 | - |
3-nitro-4-methoxybenzoate, oxygen uptake, reaction in D2O | Pseudomonas putida |
| 442.2 | - |
4-methoxybenzoate, NADH oxidation, reaction in D2O | Pseudomonas putida |
| 445 | - |
4-methoxybenzoate, NADH oxidation, reaction in H2O | Pseudomonas putida |
| 450.9 | - |
3-nitro-4-methoxybenzoate, product formation, reaction in H2O | Pseudomonas putida |
| 487.3 | - |
3-nitro-4-methoxybenzoate, product formation, reaction in D2O | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-nitro-4-methoxybenzoate + NADH + O2 | - |
Pseudomonas putida | 3-nitro-4-hydroxybenzoate + NAD+ + H2O + formaldehyde | - |
? | |
| 3-phenyl-4-methoxybenzoate + NADH + O2 | - |
Pseudomonas putida | 3-phenyl-4-hydroxybenzoate + NAD+ + H2O + formaldehyde | - |
? | |
| 4-methoxybenzoate + NADH + O2 | - |
Pseudomonas putida | 4-hydroxybenzoate + NAD+ + H2O + formaldehyde | - |
? | |
| 4-methylbenzoate + NADH + O2 | - |
Pseudomonas putida | 4-carboxybenzylalcohol + NAD+ + H2O | - |
? | |
| 4-trifluoromethylbenzoate + NADH + O2 | - |
Pseudomonas putida | ? | - |
? | |
| benzoate + NADH + O2 | - |
Pseudomonas putida | ? | - |
? | |
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