Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Putidamonooxin | - |
Pseudomonas putida |
General Stability | Organism |
---|---|
ethanol, 5-15% v/v, stabilizes activity in buffer and crude extract | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Pseudomonas putida | |
2,2'-dipyridyl | - |
Pseudomonas putida | |
5,5'-dithiobis(2-nitrobenzoate) | - |
Pseudomonas putida | |
8-hydroxyquinoline | - |
Pseudomonas putida | |
Amytal | - |
Pseudomonas putida | |
Atebrin | - |
Pseudomonas putida | |
Bathocuproinedisulfonate | - |
Pseudomonas putida | |
bathophenanthrolinedisulfate | - |
Pseudomonas putida | |
diethyldithiocarbamate | - |
Pseudomonas putida | |
EDTA | - |
Pseudomonas putida | |
KCN | - |
Pseudomonas putida | |
p-chloromercuribenzoate | reversible by GSH | Pseudomonas putida | |
rotenone | - |
Pseudomonas putida | |
Thenoyl trifluoroacetone | - |
Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
3-Nitro-4-methoxybenzoate | - |
Pseudomonas putida | |
0.0014 | - |
4-Methoxybenzoate | - |
Pseudomonas putida | |
0.008 | - |
NADH | reductase | Pseudomonas putida | |
0.077 | - |
N-methyl-4-aminobenzoate | - |
Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | both enzyme components are iron-sulfur proteins | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methoxybenzoate + NADH + O2 | Pseudomonas putida | - |
4-hydroxybenzoate + NAD+ + H2O + formaldehyde | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Oxidation Stability | Organism |
---|---|
putidamonooxin and NADH-reductase, extremely O2-sensitive, purification and storage of the reductase under anaerobic conditions | Pseudomonas putida |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 30% carbohydrate in each of the 50000 Da subunits | Pseudomonas putida |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.97 | - |
- |
Pseudomonas putida |
21.96 | - |
4-methoxybenzoate | Pseudomonas putida |
Storage Stability | Organism |
---|---|
-20°C, freeze-dried partially purified reductase stable for months without loss of activity, purified reductase is 3-4 weeks stable after addition of NADH in N2-atmosphere | Pseudomonas putida |
4°C, concentrated putidamonooxin stable for several months in N2-atmosphere with DTT and dithionite | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-nitro-4-methoxybenzoate + NADH + O2 | - |
Pseudomonas putida | 3-nitro-4-hydroxybenzoate + NAD+ + H2O + formaldehyde | - |
? | |
4-methoxybenzoate + NADH + O2 | - |
Pseudomonas putida | 4-hydroxybenzoate + NAD+ + H2O + formaldehyde | - |
? | |
N-methyl-4-aminobenzoate + NADH + O2 | - |
Pseudomonas putida | 4-aminobenzoate + NAD+ + H2O + formaldehyde | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudomonas putida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.3 | 9 | about half-maximal activity at pH 7.3 and 9.0, reductase | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Pseudomonas putida | |
NADH | - |
Pseudomonas putida |