Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Putidamonooxin | - |
Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00007 | - |
4-Methoxybenzoate | - |
Pseudomonas putida | |
0.0003 | - |
3-Nitro-4-methoxybenzoate | - |
Pseudomonas putida | |
0.0058 | - |
4-methylaminobenzoate | - |
Pseudomonas putida | |
0.009 | - |
4-Methylbenzoate | - |
Pseudomonas putida | |
0.024 | - |
4-trifluoromethylbenzoate | - |
Pseudomonas putida | |
0.029 | - |
4-hydroxybenzoate | - |
Pseudomonas putida | |
0.055 | - |
O2 | + benzoate | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | both enzyme components are iron-sulfur proteins | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41500 | - |
3-4 * 41500, putidamonooxin, SDS-PAGE | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methoxybenzoate + NADH + O2 | Pseudomonas putida | - |
4-hydroxybenzoate + NAD+ + H2O + formaldehyde | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-nitro-4-methoxybenzoate + NADH + O2 | - |
Pseudomonas putida | 3-nitro-4-hydroxybenzoate + NAD+ + H2O + formaldehyde | - |
? | |
4-hydroxybenzoate + NADH + O2 | - |
Pseudomonas putida | 3,4-dihydroxybenzoate + NAD+ + H2O | - |
? | |
4-methoxybenzoate + NADH + O2 | - |
Pseudomonas putida | 4-hydroxybenzoate + NAD+ + H2O + formaldehyde | - |
? | |
4-methylbenzoate + NADH + O2 | p-toluate, partial uncoupler | Pseudomonas putida | 4-carboxybenzylalcohol + NAD+ + H2O | in the uncoupled part of the reaction, p-toluate is not hydroxylated and H2O2 is a product, too | ? | |
4-trifluoromethylbenzoate + NADH + O2 | - |
Pseudomonas putida | ? | - |
? | |
additional information | overview: substrates that are not oxygenized while NADH-oxidation and O2-consumption are catalyzed, such as: benzoate, 3-chlorobenzoate, 4-chlorobenzoate, 2-hydroxybenzoate, 4-bromobenzoate, 2-aminobenzoate, 3-aminobenzoate, 4-trifluoromethylbenzoate, 4-tert-butylbenzoate | Pseudomonas putida | additional information | uncoupling substrates are not oxygenized, NAD+ and H2O2 being the only products of the reaction | ? | |
N-methyl-4-aminobenzoate + NADH + O2 | - |
Pseudomonas putida | 4-aminobenzoate + NAD+ + H2O + formaldehyde | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | 3-4 * 41500, putidamonooxin, SDS-PAGE | Pseudomonas putida |
oligomer | 3-4 * 33000-45000, putidamonooxin, SDS-PAGE | Pseudomonas putida |