Cloned (Comment) | Organism |
---|---|
gene CYP509C12, cloning from a cDNA library, DNA and amino acid sequence determination and analysis, phylogenetic analysis, and recombinant expression in Pichia pastoris, coexpression with NAD(P)H-dependent reductase also from Rhizopus oryzae, which improves electron transfer to CYP509C12 and thus increases the production of 11-hydroxyprogesterone by 7fold. The engineered strain displays total bioconversion of progesterone into 11alpha-hydroxyprogesterone and small amounts of 6beta-hydroxyprogesterone | Rhizopus arrhizus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | gene CYP509C12, production of a recombinant Pichia pastoris engieered to coexpress gene CYP509C12, ecoding the enzyme with the NAD(P)H-dependent reductase also from Rhizopus oryzae, to improve electron transfer to CYP509C12 and thus increases the production of 11-hydroxyprogesterone by 7fold. The engineered strain displays total bioconversion of progesterone into 11alpha-hydroxyprogesterone and small amounts of 6beta-hydroxyprogesterone and is useful for biotechnologic applications | Rhizopus arrhizus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
11-deoxycorticosterone + AH2 + O2 | Rhizopus arrhizus | - |
? + A + H2O | - |
? | |
11-deoxycorticosterone + AH2 + O2 | Rhizopus arrhizus RA 99-880 | - |
? + A + H2O | - |
? | |
11-deoxycortisol + AH2 + O2 | Rhizopus arrhizus | - |
? + A + H2O | - |
? | |
11-deoxycortisol + AH2 + O2 | Rhizopus arrhizus RA 99-880 | - |
? + A + H2O | - |
? | |
progesterone + AH2 + O2 | Rhizopus arrhizus | - |
11alpha-hydroxyprogesterone + A + H2O | - |
? | |
progesterone + AH2 + O2 | Rhizopus arrhizus RA 99-880 | - |
11alpha-hydroxyprogesterone + A + H2O | - |
? | |
testosterone + AH2 + O2 | Rhizopus arrhizus | - |
11alpha-hydroxytestosterone + A + H2O | - |
? | |
testosterone + AH2 + O2 | Rhizopus arrhizus RA 99-880 | - |
11alpha-hydroxytestosterone + A + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizopus arrhizus | - |
gene CYP509C12 | - |
Rhizopus arrhizus RA 99-880 | - |
gene CYP509C12 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11-deoxycorticosterone + AH2 + O2 | - |
Rhizopus arrhizus | ? + A + H2O | - |
? | |
11-deoxycorticosterone + AH2 + O2 | - |
Rhizopus arrhizus RA 99-880 | ? + A + H2O | - |
? | |
11-deoxycortisol + AH2 + O2 | - |
Rhizopus arrhizus | ? + A + H2O | - |
? | |
11-deoxycortisol + AH2 + O2 | - |
Rhizopus arrhizus RA 99-880 | ? + A + H2O | - |
? | |
additional information | hydroxylations take place predominantly at 11alpha and 6beta positions of the steroid ring system | Rhizopus arrhizus | ? | - |
? | |
additional information | hydroxylations take place predominantly at 11alpha and 6beta positions of the steroid ring system | Rhizopus arrhizus RA 99-880 | ? | - |
? | |
progesterone + AH2 + O2 | - |
Rhizopus arrhizus | 11alpha-hydroxyprogesterone + A + H2O | - |
? | |
progesterone + AH2 + O2 | - |
Rhizopus arrhizus RA 99-880 | 11alpha-hydroxyprogesterone + A + H2O | - |
? | |
testosterone + AH2 + O2 | - |
Rhizopus arrhizus | 11alpha-hydroxytestosterone + A + H2O | - |
? | |
testosterone + AH2 + O2 | - |
Rhizopus arrhizus RA 99-880 | 11alpha-hydroxytestosterone + A + H2O | - |
? |
Organism | Comment | Expression |
---|---|---|
Rhizopus arrhizus | CYP509C12 expression is induced by progesterone | up |