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Literature summary for 1.14.99.1 extracted from

  • Reininger, E.A.; Bauer, R.
    Prostaglandin-H-synthase (PGHS)-1 and -2 microtiter assays for the testing of herbal drugs and in vitro inhibition of PGHS-isoenzyms by polyunsaturated fatty acids from Platycodi radix (2006), Phytomedicine, 13, 164-169.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
alpha-linolenic acid
-
Ovis aries
DCM-extract of Angelicae dahuricae radix 0.1% inhibition of PGHS-1; 38.8% inhibition of PGHS-2 Ovis aries
DCM-extract of Angelicae sinsesis radix 55.8% inhibition of PGHS-2; 75.0% inhibition of PGHS-1 Ovis aries
DCM-extract of Atractylodis lanceae rhizoma 46.9% inhibition of PGHS-1; 50.3% inhibition of PGHS-2 Ovis aries
DCM-extract of Atractylodis macrocephalae rhizoma 47.0% inhibition of PGHS-2; 58.6% inhibition of PGHS-1 Ovis aries
DCM-extract of Cinnamomi ramulus 48.4% inhibition of PGHS-2; 73.5% inhibition of PGHS-1 Ovis aries
DCM-extract of Houttuyniae herba 40.9% inhibition of PGHS-2; 46.8% inhibition of PGHS-1 Ovis aries
DCM-extract of Notopterygii rhizoma seu radix -2.1% inhibition of PGHS-2; 42.6% inhibition of PGHS-1 Ovis aries
DCM-extract of Piperis sarmentosi herba 10.1% inhibition of PGHS-2; 47.2% inhibition of PGHS-1 Ovis aries
DCM-extract of Platycodi radix 70.1% inhibition of PGHS-2; 77.8% inhibition of PGHS-1 Ovis aries
DCM-extract of Zanthoxyli pericarpium 18.3% inhibition of PGHS-1; 31.3% inhibition of PGHS-2 Ovis aries
DCM-extract of Zingiberis rhizoma 41.3% inhibition of PGHS-2; 52.9% inhibition of PGHS-1 Ovis aries
indomethacin
-
Ovis aries
linoleic acid
-
Ovis aries
n-hexane extract of Angelicae dahuricae radix 42.4% inhibition of PGHS-2; 52.5% inhibition of PGHS-1 Ovis aries
n-hexane extract of Angelicae sinsesis radix 61.5% inhibition of PGHS-2; 73.0% inhibition of PGHS-1 Ovis aries
n-hexane extract of Atractylodis lanceae rhizoma 67.4% inhibition of PGHS-1; 68.3% inhibition of PGHS-2 Ovis aries
n-hexane extract of Atractylodis macrocephalae rhizoma 46.1% inhibition of PGHS-1; 48.9% inhibition of PGHS-2 Ovis aries
n-hexane extract of Cinnamomi ramulus 23.6% inhibition of PGHS-2; 46.6% inhibition of PGHS-1 Ovis aries
n-hexane extract of Houttuyniae herba 43.4% inhibition of PGHS-2; 50.3% inhibition of PGHS-1 Ovis aries
n-hexane extract of Notopterygii rhizoma seu radix 64.9% inhibition of PGHS-2; 69.6% inhibition of PGHS-1 Ovis aries
n-hexane extract of Piperis sarmentosi herba 52.4% inhibition of PGHS-1; 65.0% inhibition of PGHS-2 Ovis aries
n-hexane extract of Platycodi radix 48.7% inhibition of PGHS-1; 55.1% inhibition of PGHS-2 Ovis aries
n-hexane extract of Zanthoxyli pericarpium 24.9% inhibition of PGHS-2; 48.5% inhibition of PGHS-1 Ovis aries
n-hexane extract of Zingiberis rhizoma 77.5% inhibition of PGHS-2; 83.4% inhibition of PGHS-1 Ovis aries
NS-398
-
Ovis aries
oleic acid
-
Ovis aries

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
arachidonate + AH2 + O2 Ovis aries
-
prostaglandin H2 + A + H2O
-
?

Organism

Organism UniProt Comment Textmining
Ovis aries P05979 PGHS-1
-
Ovis aries P79208
-
-

Source Tissue

Source Tissue Comment Organism Textmining
seminal vesicle PGHS-1 Ovis aries
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidonate + AH2 + O2
-
Ovis aries prostaglandin H2 + A + H2O
-
?

Synonyms

Synonyms Comment Organism
PGHS-1
-
Ovis aries
PGHS-2
-
Ovis aries
prostaglandin-H-synthase 1
-
Ovis aries
prostaglandin-H-synthase 2
-
Ovis aries

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
activity assay at room temperature Ovis aries

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
activity assay Ovis aries

Cofactor

Cofactor Comment Organism Structure
Hematin
-
Ovis aries
additional information epinephrine-hydrogentartrate can serve as cofactor Ovis aries
additional information epinephrine-hydrogentartrate can servve as cofactor Ovis aries

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0008
-
inhibition of PGHS-2 Ovis aries indomethacin
0.0009
-
inhibition of PGHS-1 Ovis aries indomethacin
0.0026
-
inhibition of PGHS-2 Ovis aries linoleic acid
0.0026
-
inhibition of PGHS-2 Ovis aries NS-398
0.003
-
inhibition of PGHS-2 Ovis aries alpha-linolenic acid
0.0198
-
inhibition of PGHS-1 Ovis aries linoleic acid
0.0212
-
inhibition of PGHS-1 Ovis aries alpha-linolenic acid
0.0507
-
inhibition of PGHS-1 Ovis aries NS-398
0.1226
-
inhibition of PGHS-2 Ovis aries oleic acid
0.1245
-
inhibition of PGHS-1 Ovis aries oleic acid