Literature summary for 1.14.20.7 extracted from

Zhang, Z.; Smart, T.J.; Choi, H.; Hardy, F.; Lohans, C.T.; Abboud, M.I.; Richardson, M.S.W.; Paton, R.S.; McDonough, M.A.; Schofield, C.J.
Structural and stereoelectronic insights into oxygenase-catalyzed formation of ethylene from 2-oxoglutarate (2017), Proc. Natl. Acad. Sci. USA, 114, 4667-4672 .

Search for more literature for 1.14.20.7

Activating Compound

Activating Compound	Comment	Organism	Structure
ascorbate	-	Pseudomonas savastanoi pv. phaseolicola
dithiothreitol	-	Pseudomonas savastanoi pv. phaseolicola

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Pseudomonas savastanoi pv. phaseolicola

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with manganese and 2-oxoglutarate, with manganese and the buffer bis-Tris-propane, and in complex with iron, L-Arg, and N-oxalylglycine, sitting drop vapor diffusion method, using 17.5-25% (w/v) polyethylene glycol (PEG) 3350 and 6000	Pseudomonas savastanoi pv. phaseolicola

Protein Variants

Protein Variants	Comment	Organism
C317A	the mutant shows about 34% of wild type activity	Pseudomonas savastanoi pv. phaseolicola
C317S	the mutant shows about 21% of wild type activity	Pseudomonas savastanoi pv. phaseolicola
E84D	inactive	Pseudomonas savastanoi pv. phaseolicola
E84Q	inactive	Pseudomonas savastanoi pv. phaseolicola
F175Y	the mutant shows about 18% of wild type activity	Pseudomonas savastanoi pv. phaseolicola
R171A	inactive	Pseudomonas savastanoi pv. phaseolicola
R171K	inactive	Pseudomonas savastanoi pv. phaseolicola
R316A	the mutant shows about 3.7% of wild type activity	Pseudomonas savastanoi pv. phaseolicola
R316K	the mutant shows about 13% of wild type activity	Pseudomonas savastanoi pv. phaseolicola
T86S	the mutant shows about 31% of wild type activity	Pseudomonas savastanoi pv. phaseolicola
T86V	inactive	Pseudomonas savastanoi pv. phaseolicola
V270T	the mutant shows about 4.3 % of wild type activity	Pseudomonas savastanoi pv. phaseolicola
Y192F	the mutant shows about 5.6% of wild type activity	Pseudomonas savastanoi pv. phaseolicola
Y318F	the mutant shows about 65% of wild type activity	Pseudomonas savastanoi pv. phaseolicola

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	required	Pseudomonas savastanoi pv. phaseolicola

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-oxoglutarate + L-arginine + O2	Pseudomonas savastanoi pv. phaseolicola	-	succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas savastanoi pv. phaseolicola	P32021	-	-

Purification (Commentary)

Purification (Comment)	Organism
His-Trap column chromatography	Pseudomonas savastanoi pv. phaseolicola

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-oxoglutarate + L-arginine + O2	-	Pseudomonas savastanoi pv. phaseolicola	succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O	-	?

Synonyms

Synonyms	Comment	Organism
EFE	-	Pseudomonas savastanoi pv. phaseolicola
ethylene-forming enzyme	-	Pseudomonas savastanoi pv. phaseolicola

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Release 2023.1 (January 2023)