Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas syringae |
Protein Variants | Comment | Organism |
---|---|---|
H116Q | kcat value decreases to 2.4% of wild-type. Mutant is more thermolabile than wild-type | Pseudomonas syringae |
H168Q | kcat value decreases to 3% of wild-type. Mutant is more thermolabile than wild-type | Pseudomonas syringae |
H169Q | kcat value decreases to 9.3% of wild-type. Mutant is more thermolabile than wild-type | Pseudomonas syringae |
H189Q | complete loss of activity | Pseudomonas syringae |
H233Q | complete loss of activity | Pseudomonas syringae |
H268Q | kcat value decreases to 1.8% of wild-type | Pseudomonas syringae |
H284Q | kcat value decreases to 2% of wild-type. Mutant is more thermolabile than wild-type | Pseudomonas syringae |
H305Q | kcat value decreases to 40% of wild-type | Pseudomonas syringae |
H309Q | kcat value decreases to 3.3% of wild-type. Mutant is more thermolabile than wild-type | Pseudomonas syringae |
H335Q | kcat value decreases to 60% of wild-type | Pseudomonas syringae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
2-oxoglutarate | mutant H305Q, pH 8.0, 25°C | Pseudomonas syringae | |
0.007 | - |
2-oxoglutarate | mutant H168Q, pH 8.0, 25°C | Pseudomonas syringae | |
0.007 | - |
2-oxoglutarate | mutant H284Q, pH 8.0, 25°C | Pseudomonas syringae | |
0.008 | - |
2-oxoglutarate | mutant H335Q, pH 8.0, 25°C | Pseudomonas syringae | |
0.01 | - |
2-oxoglutarate | mutant H309Q, pH 8.0, 25°C | Pseudomonas syringae | |
0.011 | - |
2-oxoglutarate | mutant H169Q, pH 8.0, 25°C | Pseudomonas syringae | |
0.013 | - |
2-oxoglutarate | wild-type, pH 8.0, 25°C | Pseudomonas syringae | |
0.015 | - |
2-oxoglutarate | mutant H116Q, pH 8.0, 25°C | Pseudomonas syringae | |
0.033 | - |
2-oxoglutarate | mutant H268Q, pH 8.0, 25°C | Pseudomonas syringae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas syringae | P32021 | pv. phaseolicola PK2 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Pseudomonas syringae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + L-arginine + O2 | - |
Pseudomonas syringae | succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
2-oxoglutarate | mutant H268Q, pH 8.0, 25°C | Pseudomonas syringae | |
0.01 | - |
2-oxoglutarate | mutant H284Q, pH 8.0, 25°C | Pseudomonas syringae | |
0.012 | - |
2-oxoglutarate | mutant H116Q, pH 8.0, 25°C | Pseudomonas syringae | |
0.015 | - |
2-oxoglutarate | mutant H168Q, pH 8.0, 25°C | Pseudomonas syringae | |
0.017 | - |
2-oxoglutarate | mutant H309Q, pH 8.0, 25°C | Pseudomonas syringae | |
0.047 | - |
2-oxoglutarate | mutant H169Q, pH 8.0, 25°C | Pseudomonas syringae | |
0.2 | - |
2-oxoglutarate | mutant H305Q, pH 8.0, 25°C | Pseudomonas syringae | |
0.3 | - |
2-oxoglutarate | mutant H335Q, pH 8.0, 25°C | Pseudomonas syringae | |
0.5 | - |
2-oxoglutarate | wild-type, pH 8.0, 25°C | Pseudomonas syringae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
2-oxoglutarate | mutant H268Q, pH 8.0, 25°C | Pseudomonas syringae | |
0.8 | - |
2-oxoglutarate | mutant H116Q, pH 8.0, 25°C | Pseudomonas syringae | |
1.43 | - |
2-oxoglutarate | mutant H284Q, pH 8.0, 25°C | Pseudomonas syringae | |
1.67 | - |
2-oxoglutarate | mutant H309Q, pH 8.0, 25°C | Pseudomonas syringae | |
2.14 | - |
2-oxoglutarate | mutant H168Q, pH 8.0, 25°C | Pseudomonas syringae | |
4.24 | - |
2-oxoglutarate | mutant H169Q, pH 8.0, 25°C | Pseudomonas syringae | |
33.3 | - |
2-oxoglutarate | mutant H305Q, pH 8.0, 25°C | Pseudomonas syringae | |
37.5 | - |
2-oxoglutarate | mutant H335Q, pH 8.0, 25°C | Pseudomonas syringae | |
38.5 | - |
2-oxoglutarate | wild-type, pH 8.0, 25°C | Pseudomonas syringae |