Cloned (Comment) | Organism |
---|---|
gene ZmFLS1, ZmFLS1 is under the control of the anthocyanin (C1/PL1 + R/B) and 3-deoxy flavonoid (P1) transcriptional regulators in vivo, recombinant expression as His-tagged protein in Escherichia coli | Zea mays |
Protein Variants | Comment | Organism |
---|---|---|
H132F | site-directed mutagenesis, the mutant shows similar kinetics with dihydrokaempferol compared to the wild-type enzyme | Zea mays |
additional information | ZmFLS1 partially complements the flavonol deficiency of the Arabidopsis thaliana fls1 mutant, and restores anthocyanin accumulation to normal levels. ZmFLS1 is an immediate direct target of the P1 and C1/R regulatory complexes | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0584 | - |
dihydrokaempferol | recombinant FLS1, pH not specified in the publication, temperature not specified in the publication | Zea mays | |
0.1511 | - |
dihydroquercetin | recombinant FLS1, pH not specified in the publication, temperature not specified in the publication | Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Zea mays | FLS1 is capable of converting the dihydrokaempferol and dihydroquercetin dihydroflavonols to the corresponding flavonols, kaempferol and quercetin | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged FLS1 from Escherichia coli to about 80% purity by nickel affinity chromatography | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydrokaempferol + 2-oxoglutarate + O2 | - |
Zea mays | kaempferol + succinate + CO2 + H2O | - |
? | |
dihydroquercetin + 2-oxoglutarate + O2 | - |
Zea mays | quercetin + succinate + CO2 + H2O | - |
? | |
additional information | FLS1 is capable of converting the dihydrokaempferol and dihydroquercetin dihydroflavonols to the corresponding flavonols, kaempferol and quercetin | Zea mays | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
flavonol synthase | - |
Zea mays |
FLS1 | - |
Zea mays |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.9 | - |
dihydroquercetin | recombinant FLS1, pH not specified in the publication, temperature not specified in the publication | Zea mays | |
6.6 | - |
dihydrokaempferol | recombinant FLS1, pH not specified in the publication, temperature not specified in the publication | Zea mays |
Organism | Comment | Expression |
---|---|---|
Zea mays | the enzyme is UV-B-light-inducible in maize seedlings | up |
General Information | Comment | Organism |
---|---|---|
metabolism | ZmFLS1 is a key flavonoid biosynthetic enzyme and involved in control of flavonol accumulation in maize | Zea mays |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
26 | - |
dihydroquercetin | recombinant FLS1, pH not specified in the publication, temperature not specified in the publication | Zea mays | |
113 | - |
dihydrokaempferol | recombinant FLS1, pH not specified in the publication, temperature not specified in the publication | Zea mays |