Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbate | activity with the natural substrate (3S,5S)-carbapenam-3-carboxylate in absence of ascorbate is 2% compared to the activity in presence of ascorbate. Ascorbate does not stimulate turnover of the (3S,5R)- or (3R,5R)-stereoisomers | Pectobacterium carotovorum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required for activity | Pectobacterium carotovorum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3S,5S)-carbapenam-3-carboxylate + 2-oxoglutarate + O2 | Pectobacterium carotovorum | - |
(5R)-carbapen-2-em-3-carboxylate + succinate + CO2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pectobacterium carotovorum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3R,5R)-carbapenam-3-carboxylate + 2-oxoglutarate + O2 | activity in presence of ascorbate is 22% compared to the activity with the natural substrate (3S,5S)-carbapenam-3-carboxylate, activity in absence of ascorbate is 86% compared to the activity with the natural substrate (3S,5S)-carbapenam-3-carboxylate in presence of ascorbate | Pectobacterium carotovorum | (5R)-carbapen-2-em-3-carboxylate + succinate + CO2 + H2O | - |
? | |
(3R,5S)-carbapenam-3-carboxylate + 2-oxoglutarate + O2 | activity in presence of ascorbate is less than 2% compared to the activity with the natural substrate (3S,5S)-carbapenam, activity in absence of ascorbate is 40% compared to the activity with the natural substrate (3S,5S)-carbapenam in presence of ascorbate | Pectobacterium carotovorum | (5R)-carbapen-2-em-3-carboxylate + succinate + CO2 + H2O | - |
? | |
(3S,5R)-carbapenam-3-carboxylate + 2-oxoglutarate + O2 | activity in presence of ascorbate is 76% compared to the activity with the natural substrate (3S,5S)-carbapenam-3-carboxylate, activity in absence of ascorbate is 7% compared to the activity with the natural substrate (3S,5S)-carbapenam-3-carboxylate in presence of ascorbate | Pectobacterium carotovorum | (5R)-carbapen-2-em-3-carboxylate + succinate + CO2 + H2O | - |
? | |
(3S,5S)-carbapenam-3-carboxylate + 2-oxoglutarate + O2 | - |
Pectobacterium carotovorum | (5R)-carbapen-2-em-3-carboxylate + succinate + CO2 + H2O | - |
? | |
(3S,5S)-carbapenam-3-carboxylate + 2-oxoglutarate + O2 | natural substrate. Activity with in absence of ascorbate is 2% compared to the activity in presence of ascorbate | Pectobacterium carotovorum | (5R)-carbapen-2-em-3-carboxylate + succinate + CO2 + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CarC | - |
Pectobacterium carotovorum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
assay at | Pectobacterium carotovorum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Pectobacterium carotovorum |