Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as His-tagged fusion protein | Pseudomonas syringae pv. syringae |
General Stability | Organism |
---|---|
autooxidative destruction after a small number of turnovers, the enzyme catalyze 7 turnovers before inactivation | Pseudomonas syringae pv. syringae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the reaction requires Fe2+ | Pseudomonas syringae pv. syringae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threonyl-[L-threonyl-carrier protein SyrB1] + 2-oxoglutarate + O2 + Cl- | Pseudomonas syringae pv. syringae | the enzyme participates in syringomycin E biosynthesis | 4-chloro-L-threonyl-[L-threonyl-carrier protein SyrB1] + succinate + CO2 + H2O | - |
? | |
L-threonyl-[L-threonyl-carrier protein SyrB1] + 2-oxoglutarate + O2 + Cl- | Pseudomonas syringae pv. syringae B301D | the enzyme participates in syringomycin E biosynthesis | 4-chloro-L-threonyl-[L-threonyl-carrier protein SyrB1] + succinate + CO2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas syringae pv. syringae | Q9RBY6 | - |
- |
Pseudomonas syringae pv. syringae B301D | Q9RBY6 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas syringae pv. syringae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threonyl-[L-threonyl-carrier protein SyrB1] + 2-oxoglutarate + O2 + Cl- | the enzyme participates in syringomycin E biosynthesis | Pseudomonas syringae pv. syringae | 4-chloro-L-threonyl-[L-threonyl-carrier protein SyrB1] + succinate + CO2 + H2O | - |
? | |
L-threonyl-[L-threonyl-carrier protein SyrB1] + 2-oxoglutarate + O2 + Cl- | the enzyme can not chlorinate free L-Thr or the small molecule surrogate for L-Thr-S-SyrB1, L-Thr-S-N-acetylcysteamine | Pseudomonas syringae pv. syringae | 4-chloro-L-threonyl-[L-threonyl-carrier protein SyrB1] + succinate + CO2 + H2O | - |
? | |
L-threonyl-[L-threonyl-carrier protein SyrB1] + 2-oxoglutarate + O2 + Cl- | the enzyme participates in syringomycin E biosynthesis | Pseudomonas syringae pv. syringae B301D | 4-chloro-L-threonyl-[L-threonyl-carrier protein SyrB1] + succinate + CO2 + H2O | - |
? | |
L-threonyl-[L-threonyl-carrier protein SyrB1] + 2-oxoglutarate + O2 + Cl- | the enzyme can not chlorinate free L-Thr or the small molecule surrogate for L-Thr-S-SyrB1, L-Thr-S-N-acetylcysteamine | Pseudomonas syringae pv. syringae B301D | 4-chloro-L-threonyl-[L-threonyl-carrier protein SyrB1] + succinate + CO2 + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SyrB2 | - |
Pseudomonas syringae pv. syringae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Pseudomonas syringae pv. syringae |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme participates in syringomycin E biosynthesis | Pseudomonas syringae pv. syringae |