Literature summary for 1.14.20.15 extracted from

Fullone, M.R.; Paiardini, A.; Miele, R.; Marsango, S.; Gross, D.C.; Omura, S.; Ros-Herrera, E.; Bonaccorsi di Patti, M.C.; Lagana, A.; Pascarella, S.; Grgurina, I.
Insight into the structure-function relationship of the nonheme iron halogenases involved in the biosynthesis of 4-chlorothreonine --Thr3 from Streptomyces sp. OH-5093 and SyrB2 from Pseudomonas syringae pv. syringae B301DR (2012), FEBS J., 279, 4269-4282 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Pseudomonas syringae pv. syringae
expression in Escherichia coli	Streptomyces sp. OH-5093

Protein Variants

Protein Variants	Comment	Organism
E102A	mutation abolishes the production of syringomycin, as shown by a lack of antifungal activity	Pseudomonas syringae pv. syringae
E105A	mutation abolishes the production of syringomycin, as shown by a lack of antifungal activity	Streptomyces sp. OH-5093
F121A	mutation abolishes the production of syringomycin, as shown by a lack of antifungal activity	Pseudomonas syringae pv. syringae
F124A	mutation abolishes the production of syringomycin, as shown by a lack of antifungal activity	Streptomyces sp. OH-5093
F195A	mutation abolishes the production of syringomycin, as shown by a lack of antifungal activity	Pseudomonas syringae pv. syringae
F195A	mutation abolishes the production of syringomycin, as shown by a lack of antifungal activity	Streptomyces sp. OH-5093
F196A	inactive mutant enzyme	Pseudomonas syringae pv. syringae
F201A	inactive mutant enzyme	Streptomyces sp. OH-5093
N123A	strong decrease in antifungal activity. The residual activity is approximately 26-30%	Pseudomonas syringae pv. syringae
N126A	strong decrease in antifungal activity. The residual activity is approximately 26-30%	Streptomyces sp. OH-5093
R40A	mutant enzyme shows 26% residual activity	Pseudomonas syringae pv. syringae
R44A	mutant enzyme shows 26% residual activity	Streptomyces sp. OH-5093
Y107A	mutant enzyme shows 75% residual activity	Pseudomonas syringae pv. syringae
Y110A	mutant enzyme shows 74% residual activity	Streptomyces sp. OH-5093
Y178A	mutant enzyme shows 23-26% activity	Pseudomonas syringae pv. syringae
Y184A	mutant enzyme shows 23-26% activity	Streptomyces sp. OH-5093

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	Fe2+-dependent enzyme	Pseudomonas syringae pv. syringae
Fe2+	Fe2+-dependent enzyme	Streptomyces sp. OH-5093

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas syringae pv. syringae	Q9RBY6	-	-
Pseudomonas syringae pv. syringae B301DR	Q9RBY6	-	-
Streptomyces sp. OH-5093	H6SG30	-	-

Synonyms

Synonyms	Comment	Organism
SyrB2	-	Pseudomonas syringae pv. syringae
Thr3	-	Streptomyces sp. OH-5093

General Information

General Information	Comment	Organism
metabolism	the enzyme is involved in the biosynthesis of syringomycin	Pseudomonas syringae pv. syringae
metabolism	thr3 from Streptomyces sp. OH-5093 can replace the halogenase gene syrB2 in the biosynthesis of syringomycin, by functional complementation of the mutant Pseudomonas syringae pv. syringae strain BR135A1 inactivated in syrB2	Streptomyces sp. OH-5093

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Release 2023.1 (January 2023)