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Literature summary for 1.14.20.14 extracted from

  • Hillwig, M.L.; Liu, X.
    A new family of iron-dependent halogenases acts on freestanding substrates (2014), Nat. Chem. Biol., 10, 921-923 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information apo-WelO5 depleted of divalent metal ions can be rapidly activated aerobically with freshly prepared solutions of (NH4)2Fe(SO4)2, alpha-ketoglutarate and NaCl in a nitrogen-purged HEPES buffer Hapalosiphon welwitschii

Cloned(Commentary)

Cloned (Comment) Organism
recombinant WelO5 with an N-terminal His7 tag is overexpressed in Escherichia coli Hapalosiphon welwitschii

Protein Variants

Protein Variants Comment Organism
H259F mutant enzyme lacks chlorination activity on 12-epi-fischerindole U Hapalosiphon welwitschii

Inhibitors

Inhibitors Comment Organism Structure
Co2+ completely abolishes activity of WelO5 toward 12-epi-fischerindole U Hapalosiphon welwitschii
Cu2+ completely abolishes activity of WelO5 toward 12-epi-fischerindole U Hapalosiphon welwitschii
Mn2+ completely abolishes activity of WelO5 toward 12-epi-fischerindole U Hapalosiphon welwitschii
Ni2+ completely abolishes activity of WelO5 toward 12-epi-fischerindole U Hapalosiphon welwitschii

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ Fe2+-dependent enzyme Hapalosiphon welwitschii

Organism

Organism UniProt Comment Textmining
Hapalosiphon welwitschii A0A067YX61
-
-
Hapalosiphon welwitschii UTEX B 1830 A0A067YX61
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Hapalosiphon welwitschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
12-epi-fischerindole U + 2-oxoglutarate + O2 + chloride the rate of conversion of 12-epi-hapalindole C to 12-epi-hapalindole E by WelO5 is less than that for conversion of 12-epi-fischerindole U to 12-epi-fischerindole G. 12-epi-fischerindole U is the preferred substrate Hapalosiphon welwitschii 12-epi-fischerindole G + succinate + CO2 + H2O
-
?
12-epi-fischerindole U + 2-oxoglutarate + O2 + chloride the rate of conversion of 12-epi-hapalindole C to 12-epi-hapalindole E by WelO5 is less than that for conversion of 12-epi-fischerindole U to 12-epi-fischerindole G. 12-epi-fischerindole U is the preferred substrate Hapalosiphon welwitschii UTEX B 1830 12-epi-fischerindole G + succinate + CO2 + H2O
-
?
12-epi-hapalindole C + 2-oxoglutarate + O2 + chloride the rate of conversion of 12-epi-hapalindole C to 12-epi-hapalindole E by WelO5 is less than that for conversion of 12-epi-fischerindole U to 12-epi-fischerindole G. 12-epi-fischerindole U is the preferred substrate Hapalosiphon welwitschii 12-epi-hapalindole E + succinate + CO2 + H2O
-
?
12-epi-hapalindole C + 2-oxoglutarate + O2 + chloride the rate of conversion of 12-epi-hapalindole C to 12-epi-hapalindole E by WelO5 is less than that for conversion of 12-epi-fischerindole U to 12-epi-fischerindole G. 12-epi-fischerindole U is the preferred substrate Hapalosiphon welwitschii UTEX B 1830 12-epi-hapalindole E + succinate + CO2 + H2O
-
?

Synonyms

Synonyms Comment Organism
welO5
-
Hapalosiphon welwitschii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Hapalosiphon welwitschii

General Information

General Information Comment Organism
metabolism the enzyme is involved in welwitindolinone biosynthesis Hapalosiphon welwitschii