Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | apo-WelO5 depleted of divalent metal ions can be rapidly activated aerobically with freshly prepared solutions of (NH4)2Fe(SO4)2, alpha-ketoglutarate and NaCl in a nitrogen-purged HEPES buffer | Hapalosiphon welwitschii |
Cloned (Comment) | Organism |
---|---|
recombinant WelO5 with an N-terminal His7 tag is overexpressed in Escherichia coli | Hapalosiphon welwitschii |
Protein Variants | Comment | Organism |
---|---|---|
H259F | mutant enzyme lacks chlorination activity on 12-epi-fischerindole U | Hapalosiphon welwitschii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | completely abolishes activity of WelO5 toward 12-epi-fischerindole U | Hapalosiphon welwitschii | |
Cu2+ | completely abolishes activity of WelO5 toward 12-epi-fischerindole U | Hapalosiphon welwitschii | |
Mn2+ | completely abolishes activity of WelO5 toward 12-epi-fischerindole U | Hapalosiphon welwitschii | |
Ni2+ | completely abolishes activity of WelO5 toward 12-epi-fischerindole U | Hapalosiphon welwitschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | Fe2+-dependent enzyme | Hapalosiphon welwitschii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hapalosiphon welwitschii | A0A067YX61 | - |
- |
Hapalosiphon welwitschii UTEX B 1830 | A0A067YX61 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Hapalosiphon welwitschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
12-epi-fischerindole U + 2-oxoglutarate + O2 + chloride | the rate of conversion of 12-epi-hapalindole C to 12-epi-hapalindole E by WelO5 is less than that for conversion of 12-epi-fischerindole U to 12-epi-fischerindole G. 12-epi-fischerindole U is the preferred substrate | Hapalosiphon welwitschii | 12-epi-fischerindole G + succinate + CO2 + H2O | - |
? | |
12-epi-fischerindole U + 2-oxoglutarate + O2 + chloride | the rate of conversion of 12-epi-hapalindole C to 12-epi-hapalindole E by WelO5 is less than that for conversion of 12-epi-fischerindole U to 12-epi-fischerindole G. 12-epi-fischerindole U is the preferred substrate | Hapalosiphon welwitschii UTEX B 1830 | 12-epi-fischerindole G + succinate + CO2 + H2O | - |
? | |
12-epi-hapalindole C + 2-oxoglutarate + O2 + chloride | the rate of conversion of 12-epi-hapalindole C to 12-epi-hapalindole E by WelO5 is less than that for conversion of 12-epi-fischerindole U to 12-epi-fischerindole G. 12-epi-fischerindole U is the preferred substrate | Hapalosiphon welwitschii | 12-epi-hapalindole E + succinate + CO2 + H2O | - |
? | |
12-epi-hapalindole C + 2-oxoglutarate + O2 + chloride | the rate of conversion of 12-epi-hapalindole C to 12-epi-hapalindole E by WelO5 is less than that for conversion of 12-epi-fischerindole U to 12-epi-fischerindole G. 12-epi-fischerindole U is the preferred substrate | Hapalosiphon welwitschii UTEX B 1830 | 12-epi-hapalindole E + succinate + CO2 + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
welO5 | - |
Hapalosiphon welwitschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Hapalosiphon welwitschii |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in welwitindolinone biosynthesis | Hapalosiphon welwitschii |