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Literature summary for 1.14.20.13 extracted from
- Molecular cloning, expression and characterization of hyoscyamine 6beta-hydroxylase from hairy roots of Anisodus tanguticus (2005), Planta Med., 71, 249-253 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ascorbate | in absence of ascorbate, 14% of the activity in presence of 4 mM | Anisodus tanguticus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 5 mM, 100% inhibition | Anisodus tanguticus | |
| Cu2+ | 5 mM, 100% inhibition | Anisodus tanguticus | |
| EDTA | 5 mM, 61% inhibition | Anisodus tanguticus | |
| Fe3+ | 5 mM, 65% inhibition | Anisodus tanguticus | |
| Mg2+ | 5 mM, 14% inhibition | Anisodus tanguticus | |
| Ni2+ | 5 mM, 64% inhibition | Anisodus tanguticus | |
| Zn2+ | 5 mM, 66% inhibition | Anisodus tanguticus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.017 | - |
(6S)-6beta-hydroxyhyoscyamine | pH 7.4, 30°C | Anisodus tanguticus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | required, only 1.3% of maximum activity in absence of Fe2+ | Anisodus tanguticus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Anisodus tanguticus | Q6V0J1 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | hairy root | Anisodus tanguticus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (6S)-6beta-hydroxyhyoscyamine + 2-oxoglutarate + O2 | - |
Anisodus tanguticus | scopolamine + succinate + CO2 + H2O | - |
? | |
| additional information | enzyme catalyzes both the hydroxylation of hyoscyamine, reaction of EC 1.14.11.11, and the epoxidation of 6beta-hydroxyhyoscyamine to generate scopolamine | Anisodus tanguticus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Anisodus tanguticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Anisodus tanguticus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
20% of maximum activity | Anisodus tanguticus |
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Release 2023.1 (January 2023)
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