Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Hyoscyamus niger |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | - |
Hyoscyamus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hyoscyamus niger | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(6S)-6beta-hydroxyhyoscyamine + 2-oxoglutarate + O2 | - |
Hyoscyamus niger | scopolamine + succinate + CO2 + H2O | - |
? | |
additional information | enzyme is able to catalyze hydroxylation, dehydrogenation and in vitro epoxidation reactions all giving scopolamine. Hydroxyl rebound is unlikely to take place during the cyclization reaction and the hydroxylase versus oxidative cyclase activity is correlated with the presence of an exo-hydroxy group having syn-periplanar geometry with respect to the adjacent H atom to be abstracted | Hyoscyamus niger | ? | - |
? |