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Literature summary for 1.14.20.13 extracted from
- 6beta-hydroxyhyoscyamine epoxidase from cultured roots of Hyoscyamus niger (1989), Phytochemistry, 28, 1077-1082.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| catalase | - |
Hyoscyamus niger |
General Stability
| General Stability | Organism |
|---|---|
| rapid loss of enzyme activity during purification | Hyoscyamus niger |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CuSO4 | 100% inhibition | Hyoscyamus niger |  |
| MgSO4 | 24% inhibition | Hyoscyamus niger | |
| MnSO4 | 100% inhibition | Hyoscyamus niger | |
| additional information | no marked effect on enzyme activity by addition of NAD+, NADH, NADP+, NADPH, ATP + MgSO4, FAD, FMN, pyrroloquinoline quinone, acetyl-CoA, 6,7-dimethyl-5,6,7,8-tetrahydrofolate, phenazine methosulfate, 2,6-dichlorophenolindophenol, cytochrome c and H2O2 | Hyoscyamus niger | |
| Pyridine 2,4-dicarboxylate | - |
Hyoscyamus niger | |
| ZnSO4 | 100% inhibition | Hyoscyamus niger | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
(6S)-6-hydroxyhyoscyamine | - |
Hyoscyamus niger | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Hyoscyamus niger | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | required as co-factor | Hyoscyamus niger | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (6S)-6-hydroxyhyoscyamine + 2-oxoglutarate + O2 | Hyoscyamus niger | alkaloid metabolism, scopolamine is formed by oxidative transformation of hyoscyamine in several solanaceous species | scopolamine + succinate + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hyoscyamus niger | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| requires molecular oxygen | Hyoscyamus niger |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, could not be separated from hyoscyamine 6beta-hydroxylase | Hyoscyamus niger |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | - |
Hyoscyamus niger | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.000198 | - |
- |
Hyoscyamus niger |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, enzyme is stored in small portions until assayed | Hyoscyamus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (6S)-6-hydroxyhyoscyamine + 2-oxoglutarate + O2 | - |
Hyoscyamus niger | scopolamine + succinate + CO2 | - |
? | |
| (6S)-6-hydroxyhyoscyamine + 2-oxoglutarate + O2 | alkaloid metabolism, scopolamine is formed by oxidative transformation of hyoscyamine in several solanaceous species | Hyoscyamus niger | scopolamine + succinate + CO2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hyosOH epoxidase | - |
Hyoscyamus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Hyoscyamus niger |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoglutarate | - |
Hyoscyamus niger | |
| ascorbate | - |
Hyoscyamus niger | |
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