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Literature summary for 1.14.20.1 extracted from

  • Sim Goo, K.; Song Chua, C.; Sim, T.S.
    A complete library of amino acid alterations at R306 in Streptomyces clavuligerus deacetoxycephalosporin C synthase demonstrates its structural role in the ring-expansion activity (2008), Proteins, 70, 739-747.
    View publication on PubMed
Search for more literature for 1.14.20.1

Cloned(Commentary)

Cloned (Comment) Organism
expression as glutathione S-transferase fusion protein in Echerichia coli Streptomyces clavuligerus

Protein Variants

Protein Variants Comment Organism
R306A substrate ampicillin, 46%, penicillin G, 37%, phenethicillin, 30%, and carbenicillin, 27% of wild-type activity Streptomyces clavuligerus
R306C substrate ampicillin, 18%, penicillin G, 13%, phenethicillin, 11%, of wild-type activity Streptomyces clavuligerus
R306D substrate ampicillin, 8%, penicillin G, 4%, phenethicillin, 4%, of wild-type activity Streptomyces clavuligerus
R306E substrate ampicillin, 8%, penicillin G, 7%, phenethicillin, 6%,of wild-type activity Streptomyces clavuligerus
R306F substrate ampicillin, 55%, penicillin G, 41%, phenethicillin, 44%, and carbenicillin, 39% of wild-type activity Streptomyces clavuligerus
R306G substrate ampicillin, 21%, penicillin G, 27%, phenethicillin, 19%, of wild-type activity Streptomyces clavuligerus
R306H substrate ampicillin, 16%, penicillin G, 10%, phenethicillin, 8%, of wild-type activity Streptomyces clavuligerus
R306I substrate ampicillin, 168%, penicillin G, 113%, phenethicillin, 149%, and carbenicillin, 167% of wild-type activity Streptomyces clavuligerus
R306K substrate ampicillin, 50%, penicillin G, 53%, phenethicillin, 45%, and carbenicillin, 32% of wild-type activity Streptomyces clavuligerus
R306L substrate ampicillin, 186%, penicillin G, 105%, phenethicillin, 173%, and carbenicillin, 283% of wild-type activity Streptomyces clavuligerus
R306M substrate ampicillin, 113%, penicillin G, 102%, phenethicillin, 115%, and carbenicillin, 122% of wild-type activity Streptomyces clavuligerus
R306N substrate ampicillin, 16%, penicillin G, 14%, phenethicillin, 12%, and carbenicillin, 16% of wild-type activity Streptomyces clavuligerus
R306P no catalytic acitivity Streptomyces clavuligerus
R306Q substrate ampicillin, 62%, penicillin G, 67%, phenethicillin, 54%, and carbenicillin, 62% of wild-type activity Streptomyces clavuligerus
R306S substrate ampicillin, 39%, penicillin G, 37%, phenethicillin, 27%, and carbenicillin, 30% of wild-type activity Streptomyces clavuligerus
R306T substrate ampicillin, 40%, penicillin G, 52%, phenethicillin, 32%, and carbenicillin, 34% of wild-type activity Streptomyces clavuligerus
R306V substrate ampicillin, 34%, penicillin G, 28%, phenethicillin, 36%, and carbenicillin, 32% of wild-type activity Streptomyces clavuligerus
R306W substrate ampicillin, 90%, penicillin G, 61%, phenethicillin, 82%, and carbenicillin, 83% of wild-type activity Streptomyces clavuligerus
R306Y substrate ampicillin, 70%, penicillin G, 59%, phenethicillin, 61%, and carbenicillin, 60% of wild-type activity Streptomyces clavuligerus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.23
-
 ampicillin mutant R306M, 30°C Streptomyces clavuligerus
8.33
-
 ampicillin wild-type, 30°C Streptomyces clavuligerus
9.11
-
 ampicillin mutant R306I, 30° Streptomyces clavuligerus
10.9
-
 ampicillin mutant R306L, 30°C Streptomyces clavuligerus

Organism

Organism UniProt Comment Textmining
Streptomyces clavuligerus P18548
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.41
-
 substrate ampicillin, wild-type, 30°C Streptomyces clavuligerus
7.23
-
 substrate ampicillin, mutant R306M, 30°C Streptomyces clavuligerus
9.11
-
 substrate ampicillin, mutant R306I, 30° Streptomyces clavuligerus
10.9
-
 substrate ampicillin, mutant R306L, 30°C Streptomyces clavuligerus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ampicillin + 2-oxoglutarate + O2
-
 Streptomyces clavuligerus ? + succinate + CO2
-
 ?
carbenicillin + 2-oxoglutarate + O2
-
 Streptomyces clavuligerus ? + succinate + CO2
-
 ?
penicillin G + 2-oxoglutarate + O2
-
 Streptomyces clavuligerus ? + succinate + CO2
-
 ?
phenethicillin + 2-oxoglutarate + O2
-
 Streptomyces clavuligerus ? + succinate + CO2
-
 ?