Literature summary for 1.14.20.1 extracted from

Shen, Y.Q.; Wolfe, S.; Demain, A.L.
Desacetoxycephalosporin C synthetase: importance of order of cofactor/reactant addition (1984), Enzyme Microb. Technol., 6, 402-404.

No PubMed abstract available

Search for more literature for 1.14.20.1

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	best activity is obtained by adding to the enzyme a mixture of ATP, Fe2+, and ascorbate, followed by addition of 2-oxoglutarate, and then penicillin N. Simultaneous addition of ATP, Fe2+, and ascorbate is preferable to sequential addition	Acremonium chrysogenum

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	when 2-oxoglutarate is added to the enzyme before the mixture of ATP, Fe2+ and ascobate activity is only about 40% of the reaction	Acremonium chrysogenum

Organism

Organism	UniProt	Comment	Textmining
Acremonium chrysogenum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Acremonium chrysogenum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
penicillin N + 2-oxoglutarate + O2	almost absolute requirement for 2-oxoglutarate	Acremonium chrysogenum	deacetoxycephalosporin C + succinate + CO2 + H2O	-	?

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Release 2023.1 (January 2023)