Cloned (Comment) | Organism |
---|---|
overexpression as an insoluble and inactive enzyme in granules of recombinant Escherichia coli | Acremonium chrysogenum |
Organic Solvent | Comment | Organism |
---|---|---|
urea | purification is achieved by an anion-exchange-chromatographic step in the presence of denaturing concentrations of urea. The main obstacle to converting the homogenous unfolded protein into the active enzyme is a urea-dependent aggregation during refolding that led to irreversible enzyme inactivation | Acremonium chrysogenum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acremonium chrysogenum | - |
bifunctional enzyme deacetoxycephalosporin C synthetase/hydroxylase | - |
Purification (Comment) | Organism |
---|---|
purification of refolding-competent recombinant enzyme | Acremonium chrysogenum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.264 | - |
- |
Acremonium chrysogenum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
penicillin N + 2-oxoglutarate + O2 | - |
Acremonium chrysogenum | deacetoxycephalosporin C + succinate + CO2 + H2O | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
at pH 10 enzyme has similar stability at 4°C and at 25°C. At pH 11 the enzyme is more stable at 4°C than at 25°C | Acremonium chrysogenum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
10 | - |
enzyme has similar stability at 4°C and at 25°C | Acremonium chrysogenum |
11 | - |
enzyme is more stable at 4°C than at 25°C | Acremonium chrysogenum |