Cloned (Comment) | Organism |
---|---|
- |
Lentzea aerocolonigenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | stopped-flow analysis of flavin intermediates | Lentzea aerocolonigenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Lentzea aerocolonigenes | first step in biosynthesis of rebeccamycin | ? | - |
? | |
tryptophan + FADH2 + O2 + Cl- + H+ | Lentzea aerocolonigenes | initial step of rebeccamycin biosynthesis | 7-chlorotryptophan + FAD + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lentzea aerocolonigenes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
RebH is purified by size-exclusion chromatography | Lentzea aerocolonigenes |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
tryptophan + FADH2 + chloride + O2 + H+ = 7-chloro-L-tryptophan + FAD + 2 H2O | FADH2 and O2 form an 4alpha-FAD-OOH intermediate that decays to 4alpha-FAD-OH. Tryptophan likely does not react directly with any flavin intermediate. Substrate chlorination occurs after completion of the flavin redox reactions | Lentzea aerocolonigenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | first step in biosynthesis of rebeccamycin | Lentzea aerocolonigenes | ? | - |
? | |
tryptophan + FADH2 + O2 + Cl- + H+ | initial step of rebeccamycin biosynthesis | Lentzea aerocolonigenes | 7-chlorotryptophan + FAD + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
halogenase | - |
Lentzea aerocolonigenes |
RebH | - |
Lentzea aerocolonigenes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
end-point assay for single-turnover reactions | Lentzea aerocolonigenes |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
FADH2 | 4°C | Lentzea aerocolonigenes | |
0.05 | - |
tryptophan | ranges from 0.02 s-1 to 0.05 s-1 for different preparations, 25°C | Lentzea aerocolonigenes | |
0.12 | - |
FADH2 | 25°C | Lentzea aerocolonigenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
end-point assay for single-turnover reactions | Lentzea aerocolonigenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FADH2 | - |
Lentzea aerocolonigenes |