Cloned (Comment) | Organism |
---|---|
heterologous expression of wild-type enzyme and mutant enzymes A308S and A308T in Sf-9 cells | Sesamum indicum |
Protein Variants | Comment | Organism |
---|---|---|
A308S | the mutant enzyme exhibits comparable substrate specificity (Km) to those of wild-type, and its catalytic activity (kcat) is moderately decreased to approximately one-third of wild-type value | Sesamum indicum |
A308T | compared to those of wild-type enzyme, the Km-value is increased to 6fold and the kcat is decreased to approximately 52fold | Sesamum indicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(+)-kobusin | - |
Sesamum indicum | |
(+)-pinoresinol | - |
Sesamum indicum | |
5-[(1S,3aS,4R,6aR)-4-(1,3-benzodioxol-5-yl)tetrahydro-1H,3H-furo[3,4-c]furan-1-yl]-2-methoxyphenol | - |
Sesamum indicum | |
additional information | no inhibition by (+)-epipinoresinol and (-)-pinoresinol | Sesamum indicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
SDS-PAGE | Sesamum indicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-pinoresinol + [reduced NADPH-hemoprotein reductase] + O2 | Sesamum indicum | - |
(+)-piperitol + [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? | |
(+)-piperitol + [reduced NADPH-hemoprotein reductase] + O2 | Sesamum indicum | - |
(+)-sesamin + [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sesamum indicum | Q33DY0 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-pinoresinol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Sesamum indicum | (+)-piperitol + [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? | |
(+)-piperitol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Sesamum indicum | (+)-sesamin + [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? | |
additional information | no activity with (+)-phylligenin, (+)-epipinoresinol, (+)-pinoresinol 4'-O-glucoside, (+)-pinoreinol monomethylether, (+)-kobusin, (+)-syringaresinol, (-)-syringaresinol, (-)-lariciresinol, (-)-pinoresinol, (-)-matairesinol or (+)-sesamolinol | Sesamum indicum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE | Sesamum indicum |
Synonyms | Comment | Organism |
---|---|---|
CYP81Q1 | - |
Sesamum indicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P-450 | a cytochrome P-450 (heme-thiolate) protein | Sesamum indicum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0198 | - |
5-[(1S,3aS,4R,6aR)-4-(1,3-benzodioxol-5-yl)tetrahydro-1H,3H-furo[3,4-c]furan-1-yl]-2-methoxyphenol | pH and temperature not specified in the publication | Sesamum indicum |