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Literature summary for 1.14.19.72 extracted from

  • Marques, J.V.; Kim, K.W.; Lee, C.; Costa, M.A.; May, G.D.; Crow, J.A.; Davin, L.B.; Lewis, N.G.
    Next generation sequencing in predicting gene function in podophyllotoxin biosynthesis (2013), J. Biol. Chem., 288, 466-479.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.19.72

Cloned(Commentary)

Cloned (Comment) Organism
gene CYP719A23, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis Sinopodophyllum hexandrum
gene CYP719A24, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis Podophyllum peltatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state Michaelis-Menten kinetics Sinopodophyllum hexandrum
additional information
-
 additional information steady-state Michaelis-Menten kinetics Podophyllum peltatum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(-)-matairesinol + [reduced NADPH-hemoprotein reductase] + O2 Sinopodophyllum hexandrum
-
 (-)-pluviatolide + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
(-)-matairesinol + [reduced NADPH-hemoprotein reductase] + O2 Podophyllum peltatum
-
 (-)-pluviatolide + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
additional information Sinopodophyllum hexandrum the enzyme is capable of converting (-)-matairesinol into (-)-pluviatolide by catalyzing methylenedioxy bridge formation and does not act on other possible substrates tested, i.e. (+)-pinoresinol, (-)-pinoresinol, (-)-arctigenin, (-)-alpha-conidendrin, (+)-piperitol, (-)-piperitol, and (+)-phillygenin ?
-
 ?
additional information Podophyllum peltatum the enzyme is capable of converting (-)-matairesinol into (-)-pluviatolide by catalyzing methylenedioxy bridge formation and does not act on other possible substrates tested, i.e. (+)-pinoresinol, (-)-pinoresinol, (-)-arctigenin, (-)-alpha-conidendrin, (+)-piperitol, (-)-piperitol, and (+)-phillygenin ?
-
 ?

Organism

Organism UniProt Comment Textmining
Podophyllum peltatum L7T720 gene CYP719A24
-
Sinopodophyllum hexandrum L7T8H2 gene CYP719A23
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(-)-matairesinol + [reduced NADPH-hemoprotein reductase] + O2
-
 Sinopodophyllum hexandrum (-)-pluviatolide + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
(-)-matairesinol + [reduced NADPH-hemoprotein reductase] + O2
-
 Podophyllum peltatum (-)-pluviatolide + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
(-)-matairesinol + [reduced NADPH-hemoprotein reductase] + O2 (-)-matairesinol is isolated from Forsythia intermedia Sinopodophyllum hexandrum (-)-pluviatolide + [oxidized NADPH-hemoprotein reductase] + H2O product identification and quantification by 1H, 13C, and 13C,1H heteronuclear single quantum coherence NMR spectroscopic analyses ?
(-)-matairesinol + [reduced NADPH-hemoprotein reductase] + O2 (-)-matairesinol is isolated from Forsythia intermedia Podophyllum peltatum (-)-pluviatolide + [oxidized NADPH-hemoprotein reductase] + H2O product identification and quantification by 1H, 13C, and 13C,1H heteronuclear single quantum coherence NMR spectroscopic analyses ?
additional information the enzyme is capable of converting (-)-matairesinol into (-)-pluviatolide by catalyzing methylenedioxy bridge formation and does not act on other possible substrates tested, i.e. (+)-pinoresinol, (-)-pinoresinol, (-)-arctigenin, (-)-alpha-conidendrin, (+)-piperitol, (-)-piperitol, and (+)-phillygenin Sinopodophyllum hexandrum ?
-
 ?
additional information the enzyme is capable of converting (-)-matairesinol into (-)-pluviatolide by catalyzing methylenedioxy bridge formation and does not act on other possible substrates tested, i.e. (+)-pinoresinol, (-)-pinoresinol, (-)-arctigenin, (-)-alpha-conidendrin, (+)-piperitol, (-)-piperitol, and (+)-phillygenin Podophyllum peltatum ?
-
 ?

Synonyms

Synonyms Comment Organism
CYP719A23
-
 Sinopodophyllum hexandrum
CYP719A24
-
 Podophyllum peltatum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Sinopodophyllum hexandrum
25
-
 assay at Podophyllum peltatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Sinopodophyllum hexandrum
7.5
-
 assay at Podophyllum peltatum

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
 Sinopodophyllum hexandrum
cytochrome P450
-
 Podophyllum peltatum

General Information

General Information Comment Organism
metabolism the enzyme is involved in the podophyllotoxin biosynthesis, detailed pathway overview Sinopodophyllum hexandrum
metabolism the enzyme is involved in the podophyllotoxin biosynthesis, detailed pathway overview Podophyllum peltatum