Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.19.70 extracted from

  • Fonvielle, M.; Le Du, M.H.; Lequin, O.; Lecoq, A.; Jacquet, M.; Thai, R.; Dubois, S.; Grach, G.; Gondry, M.; Belin, P.
    Substrate and reaction specificity of Mycobacterium tuberculosis cytochrome P450 CYP121: insights from biochemical studies and crystal structures (2013), J. Biol. Chem., 288, 17347-17359.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.19.70

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
ligand-bound enzyme, sitting drop vapor diffusion method, using in 100 mM Na-MES bufferat pH 5.0-5.5 and 1.6-2.2 M (NH4)2SO4, at 6°C Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
(3S,6S)-3,6-bis(4-hydroxybenzyl)piperazin-2-one
-
 Mycobacterium tuberculosis
cyclo(L-tyrosyl-L-alanyl)
-
 Mycobacterium tuberculosis
cyclo(L-tyrosyl-L-phenylalanyl)
-
 Mycobacterium tuberculosis
cyclo(L-tyrosyl-L-tryptophanyl)
-
 Mycobacterium tuberculosis
cyclo-(L-tyrosyl-DOPA)
-
 Mycobacterium tuberculosis
additional information not inhibited by cyclo(L-Tyr-L-Leu) and cyclo(L-Tyr-L-Met) Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WPP7
-
-
Mycobacterium tuberculosis H37Rv P9WPP7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3S,6S)-3,6-bis(4-hydroxybenzyl)piperazin-2-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 98% of the compound remains after 1 h incubation with CYP121 Mycobacterium tuberculosis ?
-
 ?
(3S,6S)-3,6-bis(4-hydroxybenzyl)piperazin-2-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 98% of the compound remains after 1 h incubation with CYP121 Mycobacterium tuberculosis H37Rv ?
-
 ?
cyclo(L-tyrosyl-L-phenylalanyl) + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 the rate of transformation of cyclo(L-tyrosyl-L-phenylalanyl) is very slow, with about 98% of compound remaining after 1 h of incubation with CYP121 Mycobacterium tuberculosis ?
-
 ?
cyclo(L-tyrosyl-L-phenylalanyl) + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 the rate of transformation of cyclo(L-tyrosyl-L-phenylalanyl) is very slow, with about 98% of compound remaining after 1 h of incubation with CYP121 Mycobacterium tuberculosis H37Rv ?
-
 ?
cyclo(L-tyrosyl-L-tryptophanyl) + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 about 50% of the initial compound remains after a 1 h incubation with CYP121 Mycobacterium tuberculosis ?
-
 ?
cyclo(L-tyrosyl-L-tryptophanyl) + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 about 50% of the initial compound remains after a 1 h incubation with CYP121 Mycobacterium tuberculosis H37Rv ?
-
 ?
cyclo(L-tyrosyl-L-tyrosyl) + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 100% transformation Mycobacterium tuberculosis mycocyclosin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
cyclo(L-tyrosyl-L-tyrosyl) + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 100% transformation Mycobacterium tuberculosis H37Rv mycocyclosin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
cyclo-(L-tyrosyl-DOPA) + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 transformation of cyclo-(L-tyrosyl-DOPA) by CYP121 is very slow, 91% of the compound remains after 1 h of incubation with CYP121 Mycobacterium tuberculosis ?
-
 ?
additional information no activity with cyclo(L-tyrosyl-L-alanyl) Mycobacterium tuberculosis ?
-
 ?
additional information no activity with cyclo(L-tyrosyl-L-alanyl) Mycobacterium tuberculosis H37Rv ?
-
 ?

Synonyms

Synonyms Comment Organism
CYP121
-
 Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
cytochrome P450 CYP121 is a highly specific cytochrome P450 Mycobacterium tuberculosis