Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) pLysS cells | Streptomyces coelicolor |
Crystallization (Comment) | Organism |
---|---|
I87K mutant of isoform CYP158A2, hanging drop vapor diffusion method, using 0.1 M bis-Tris (pH 6.5), 1.2 M ammonium dihydrogen phosphate | Streptomyces coelicolor |
Protein Variants | Comment | Organism |
---|---|---|
I87K | mutant of isoform CYP158A2, the mutation significantly changes the ratios of the dimerization products converting CYP158A2 into a CYP158A1-like monooxygenase | Streptomyces coelicolor |
K90I | mutant of isoform CYP158A1, the catalytic activity of the mutant does not change at all compared with the wild type enzyme | Streptomyces coelicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0073 | - |
flaviolin | wild type isoform CYP158A2, at pH 8.2 and 37°C | Streptomyces coelicolor | |
0.0105 | - |
flaviolin | wild type isoform CYP158A1, at pH 8.2 and 37°C | Streptomyces coelicolor | |
0.0169 | - |
flaviolin | mutant K90I of isoform CYP158A1, at pH 8.2 and 37°C | Streptomyces coelicolor | |
0.0432 | - |
flaviolin | mutant I87K of isoform CYP158A2, at pH 8.2 and 37°C | Streptomyces coelicolor |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 flaviolin + 4 reduced ferredoxin [iron-sulfur] cluster + 4 H+ + O2 | Streptomyces coelicolor | CYP158A2 produces three isomers of biflaviolin and one triflaviolin | 3,3'-biflaviolin + 3,8'-biflaviolin + 4 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O | - |
? | |
4 flaviolin + 4 reduced ferredoxin [iron-sulfur] cluster + 4 H+ + O2 | Streptomyces coelicolor | isoform CYP158A1 can only produce 3,3'-biflaviolin and 3,8'-biflaviolin with quite different molar ratios compared with the products from CYP158A2 | 3,3'-biflaviolin + 3,8'-biflaviolin + 4 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O | - |
? | |
4 flaviolin + 4 reduced ferredoxin [iron-sulfur] cluster + 4 H+ + O2 | Streptomyces coelicolor A3(2) | CYP158A2 produces three isomers of biflaviolin and one triflaviolin | 3,3'-biflaviolin + 3,8'-biflaviolin + 4 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O | - |
? | |
4 flaviolin + 4 reduced ferredoxin [iron-sulfur] cluster + 4 H+ + O2 | Streptomyces coelicolor A3(2) | isoform CYP158A1 can only produce 3,3'-biflaviolin and 3,8'-biflaviolin with quite different molar ratios compared with the products from CYP158A2 | 3,3'-biflaviolin + 3,8'-biflaviolin + 4 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces coelicolor | Q9FCA6 | - |
- |
Streptomyces coelicolor | Q9KZF5 | - |
- |
Streptomyces coelicolor A3(2) | Q9FCA6 | - |
- |
Streptomyces coelicolor A3(2) | Q9KZF5 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+ affinity chromatography and S-Sepharose/Q-Sepharose column chromatography | Streptomyces coelicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 flaviolin + 4 reduced ferredoxin [iron-sulfur] cluster + 4 H+ + O2 | CYP158A2 produces three isomers of biflaviolin and one triflaviolin | Streptomyces coelicolor | 3,3'-biflaviolin + 3,8'-biflaviolin + 4 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O | - |
? | |
4 flaviolin + 4 reduced ferredoxin [iron-sulfur] cluster + 4 H+ + O2 | isoform CYP158A1 can only produce 3,3'-biflaviolin and 3,8'-biflaviolin with quite different molar ratios compared with the products from CYP158A2 | Streptomyces coelicolor | 3,3'-biflaviolin + 3,8'-biflaviolin + 4 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O | - |
? | |
4 flaviolin + 4 reduced ferredoxin [iron-sulfur] cluster + 4 H+ + O2 | CYP158A2 produces three isomers of biflaviolin and one triflaviolin | Streptomyces coelicolor A3(2) | 3,3'-biflaviolin + 3,8'-biflaviolin + 4 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O | - |
? | |
4 flaviolin + 4 reduced ferredoxin [iron-sulfur] cluster + 4 H+ + O2 | isoform CYP158A1 can only produce 3,3'-biflaviolin and 3,8'-biflaviolin with quite different molar ratios compared with the products from CYP158A2 | Streptomyces coelicolor A3(2) | 3,3'-biflaviolin + 3,8'-biflaviolin + 4 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP158A1 | isoform | Streptomyces coelicolor |
CYP158A2 | isoform | Streptomyces coelicolor |