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Literature summary for 1.14.19.68 extracted from

  • Rueffer, M.; Zenk, M.H.
    Canadine synthase from Thalictrum tuberosum cell cultures catalyses the formation of the methylenedioxy bridge in berberine synthesis (1994), Phytochemistry, 36, 1219-1223.
No PubMed abstract available
Search for more literature for 1.14.19.68

Inhibitors

Inhibitors Comment Organism Structure
cytochrome c microsomal-bound enzyme Thalictrum tuberosum
juglone microsomal-bound enzyme Thalictrum tuberosum
ketoconazole microsomal-bound enzyme Thalictrum tuberosum
menadione microsomal-bound enzyme Thalictrum tuberosum
Metyrapone microsomal-bound enzyme Thalictrum tuberosum
Plumbagin microsomal-bound enzyme Thalictrum tuberosum
Prochloraz microsomal-bound enzyme Thalictrum tuberosum
Propiconazole microsomal-bound enzyme Thalictrum tuberosum
Tetcyclacis microsomal-bound enzyme Thalictrum tuberosum
Triadimefone microsomal-bound enzyme Thalictrum tuberosum
tropolone microsomal-bound enzyme Thalictrum tuberosum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0115
-
 (S)-tetrahydrocolumbamine canadine synthase in complex with a cytochrome P-450 reductase Thalictrum tuberosum
0.033
-
 [reduced NADPH-hemoprotein reductase] canadine synthase in complex with a cytochrome P-450 reductase Thalictrum tuberosum

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Thalictrum tuberosum
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2 Thalictrum tuberosum penultimate step in the biosynthesis of the protoberberine alkaloid, berberine (S)-canadine + [oxidized NADPH-hemoprotein reductase] + H2O (S)-canadine is identical with (S)-tetrahydroberberine ?

Organism

Organism UniProt Comment Textmining
Berberis aristata
-
-
-
Berberis crataegina
-
-
-
Berberis henryana
-
-
-
Berberis stolonifera
-
-
-
Berberis taliensis
-
-
-
Coptis japonica
-
-
-
Thalictrum glabrum
-
-
-
Thalictrum glaucum
-
-
-
Thalictrum macrocarpum
-
-
-
Thalictrum tuberosum
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Coptis japonica
-
cell culture protoberberine producing cell line Thalictrum tuberosum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2 high substrate specificity Thalictrum tuberosum (S)-canadine + [oxidized NADPH-hemoprotein reductase] + H2O (S)-canadine is identical with (S)-tetrahydroberberine ?
(S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2 penultimate step in the biosynthesis of the protoberberine alkaloid, berberine Thalictrum tuberosum (S)-canadine + [oxidized NADPH-hemoprotein reductase] + H2O (S)-canadine is identical with (S)-tetrahydroberberine ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 canadine synthase in complex with a cytochrome P-450 reductase Thalictrum tuberosum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 canadine synthase in complex with a cytochrome P-450 reductase Thalictrum tuberosum

Cofactor

Cofactor Comment Organism Structure
cytochrome P450 the enzyme forms a complex with a cytochrome P-450 reductase Thalictrum tuberosum