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Literature summary for 1.14.19.65 extracted from

  • Bauer, W.; Zenk, M.H.
    Two methylenedioxy bridge forming cytochrome P-450 dependent enzymes are involved in (S)-stylopine biosynthesis (1991), Phytochemistry, 30, 2953-2961.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0009
-
(S)-scoulerine
-
Eschscholzia californica

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Eschscholzia californica
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-scoulerine + [reduced NADPH-hemoprotein reductase] + O2 Eschscholzia californica enzyme is induced 20 h after challenging the cell suspension cultur with elicitor (S)-cheilanthifoline + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Organism

Organism UniProt Comment Textmining
Eschscholzia californica
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
Eschscholzia californica
-

Storage Stability

Storage Stability Organism
-20°C, 15% loss of activity after 4 months Eschscholzia californica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-scoulerine + [reduced NADPH-hemoprotein reductase] + O2 NADPH is essential for activity, NADH displays only 0.5% turnover of that of NADPH Eschscholzia californica (S)-cheilanthifoline + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
(S)-scoulerine + [reduced NADPH-hemoprotein reductase] + O2 enzyme is induced 20 h after challenging the cell suspension cultur with elicitor Eschscholzia californica (S)-cheilanthifoline + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Eschscholzia californica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
half-life: 30 h Eschscholzia californica
25
-
half-life: 2.5 h Eschscholzia californica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Eschscholzia californica

pH Range

pH Minimum pH Maximum Comment Organism
7 9 about 50% of maximal activity at pH 7 and pH 9 Eschscholzia californica

Cofactor

Cofactor Comment Organism Structure
cytochrome P450 the enzyme is a cytochrome P450 dependent monooxygenase Eschscholzia californica
FAD 0.004 mM, together with the optimal concentration of NADPH, 0.2 mM, enhances activity by 50% Eschscholzia californica
FMN 0.004 mM, together with the optimal concentration of NADPH, 0.2 mM, enhances activity by 50% Eschscholzia californica