Reference to 1.14.19.62; Id = 744599 - BRENDA Enzyme Database

gene CYP72C, DNA and amino acid sequence determination and analysis, sequence comparisons, transcriptome analysis, real-time PCR expression analysis

Catharanthus roseus

endoplasmic reticulum

an endoplasmic reticulum-anchored enzyme

Catharanthus roseus

5783

-

loganin + [reduced NADPH-hemoprotein reductase] + O2

Catharanthus roseus

-

secologanin + [oxidized NADPH-hemoprotein reductase] + 2 H2O

-

?

additional information

Catharanthus roseus

the second secologanin synthase isoform SLS2 produces both secologanin and secoxyloganin

?

-

?

Catharanthus roseus

U5NDT8

-

additional information

SLS2 might be the prominent isoform of secologanin biosynthesis in the aerial parts of the plant

Catharanthus roseus

-

loganin + [reduced NADPH-hemoprotein reductase] + O2

-

744599

Catharanthus roseus

secologanin + [oxidized NADPH-hemoprotein reductase] + 2 H2O

-

?

additional information

the second secologanin synthase isoform SLS2 produces both secologanin and secoxyloganin

744599

Catharanthus roseus

?

-

?

CYP72C

-

Catharanthus roseus

SLS

-

Catharanthus roseus

30

-

assay at

Catharanthus roseus

7.5

-

assay at

Catharanthus roseus

cytochrome P450

-

Catharanthus roseus

NADPH-hemoprotein reductase

-

Catharanthus roseus

gene CYP72C, DNA and amino acid sequence determination and analysis, sequence comparisons, transcriptome analysis, real-time PCR expression analysis

Catharanthus roseus

cytochrome P450

-

Catharanthus roseus

NADPH-hemoprotein reductase

-

Catharanthus roseus

endoplasmic reticulum

an endoplasmic reticulum-anchored enzyme

Catharanthus roseus

5783

-

loganin + [reduced NADPH-hemoprotein reductase] + O2

Catharanthus roseus

-

secologanin + [oxidized NADPH-hemoprotein reductase] + 2 H2O

-

?

additional information

Catharanthus roseus

the second secologanin synthase isoform SLS2 produces both secologanin and secoxyloganin

?

-

?

additional information

SLS2 might be the prominent isoform of secologanin biosynthesis in the aerial parts of the plant

Catharanthus roseus

-

loganin + [reduced NADPH-hemoprotein reductase] + O2

-

744599

Catharanthus roseus

secologanin + [oxidized NADPH-hemoprotein reductase] + 2 H2O

-

?

additional information

the second secologanin synthase isoform SLS2 produces both secologanin and secoxyloganin

744599

Catharanthus roseus

?

-

?

30

-

assay at

Catharanthus roseus

7.5

-

assay at

Catharanthus roseus

metabolism

the enzyme is important in the monoterpenoid indole alkaloid (MIA) biosynthesis in Catharanthus roseus, subcellular organization of the central steps of the pathway, overview. The ring-opening reaction of loganin in the biosynthesis of secologanin is catalyzed by the fourth P450 of this pathway, secologanin synthase (SLS, CYP72A1). Both isozymes SLS1 and SLS2 not only catalyze the oxidative ring cleavage of loganin to produce secologanin but also perform the oxidation of secologanin into secoxyloganin. SLS1 and SLS2 constitute the third type of P450 from the seco-iridoid pathway performing more than one catalytic reaction. Additional enzymes might convert secoxyloganin back to secologanin, the subcellular compartmentation of secologanin biosynthesis may also limit secoxyloganin formation in planta. Secoxyloganin is an acidic compound derived from secologanin that is no longer able to be condensed with tryptamine by STR in the vacuole, due to the absence of the aldehyde function. If secoxyloganin formation occurs in vivo, the resulting depletion of the secologanin pool is deleterious for the subsequent synthesis of MIAs

Catharanthus roseus

additional information

consensus transcriptome analysis of Catharanthus roseus, detailed overview

Catharanthus roseus

physiological function

secologanin synthases (SLS) are P450 enzymes that catalyze an unusual ring-opening reaction of loganin in the biosynthesis of the monoterpenoid indole alkaloids (MIA) precursor secologanin

Catharanthus roseus

metabolism

the enzyme is important in the monoterpenoid indole alkaloid (MIA) biosynthesis in Catharanthus roseus, subcellular organization of the central steps of the pathway, overview. The ring-opening reaction of loganin in the biosynthesis of secologanin is catalyzed by the fourth P450 of this pathway, secologanin synthase (SLS, CYP72A1). Both isozymes SLS1 and SLS2 not only catalyze the oxidative ring cleavage of loganin to produce secologanin but also perform the oxidation of secologanin into secoxyloganin. SLS1 and SLS2 constitute the third type of P450 from the seco-iridoid pathway performing more than one catalytic reaction. Additional enzymes might convert secoxyloganin back to secologanin, the subcellular compartmentation of secologanin biosynthesis may also limit secoxyloganin formation in planta. Secoxyloganin is an acidic compound derived from secologanin that is no longer able to be condensed with tryptamine by STR in the vacuole, due to the absence of the aldehyde function. If secoxyloganin formation occurs in vivo, the resulting depletion of the secologanin pool is deleterious for the subsequent synthesis of MIAs

Catharanthus roseus

additional information

consensus transcriptome analysis of Catharanthus roseus, detailed overview

Catharanthus roseus

physiological function

secologanin synthases (SLS) are P450 enzymes that catalyze an unusual ring-opening reaction of loganin in the biosynthesis of the monoterpenoid indole alkaloids (MIA) precursor secologanin

Catharanthus roseus

744599

Duge de Bernonville

Characterization of a second ...

Catharanthus roseus

BMC Genomics

16

619

2015

-

1

-

1

-

2

-

4

-

1

-

2

-

2

1

-

1

-

2

-

1

2

-

1

-

2

-

1

-

2

-

1

-

1

-

3

-

724967

Guirimand

The subcellular organization o ...

Catharanthus roseus

FEBS J.

278

749-763

2011

-

1

-

1

-

1

-

4

-

4

-

1

-

3

-

1

-

1

-

1

-

1

-

4

-

1

-

3

-

670558

Murata

localization of tabersonine 16 ...

Catharanthus roseus

Plant J.

44

581-594

2005

-

1

-

3

-

2

-

1

-

2

-

437785

Yamamoto

Secologanin synthase which cat ...

Lonicera japonica

Phytochemistry

53

7-12

2000

-

5

-

1

-

1

-

3

-

1

-

2

-

3

-

1

-

1

-

5

-

1

-

1

-

2

-

3

-

1

-

1

-

437786

Irmler

Indole alkaloid biosynthesis i ...

Catharanthus roseus

Plant J.

24

797-804

2000

-

1

-

1

-

7

-

1

-

1

-

3

-

1

-

1

-

1

-

1

-

3

-

437784

Yamamoto

-

Transformation of loganin and ...

Lonicera japonica

Phytochemistry

50

417-422

1999

-

1

-

1

-

1

-

1

-

2

-

1

-

1

-

2

-

Characterization of a second secologanin synthase isoform producing both secologanin and secoxyloganin allows enhanced de novo assembly of a Catharanthus roseus transcriptome

Data extracted from this reference: