Literature summary for 1.14.19.62 extracted from

Duge de Bernonville, T.; Foureau, E.; Parage, C.; Lanoue, A.; Clastre, M.; Londono, M.A.; Oudin, A.; Houille, B.; Papon, N.; Besseau, S.; Glevarec, G.; Atehortua, L.; Giglioli-Guivarch, N.; St-Pierre, B.; De Luca, V.; O'Connor, S.E.; Courdavault, V.
Characterization of a second secologanin synthase isoform producing both secologanin and secoxyloganin allows enhanced de novo assembly of a Catharanthus roseus transcriptome (2015), BMC Genomics, 16, 619 .

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Cloned(Commentary)

Cloned (Comment)	Organism
gene CYP72C, DNA and amino acid sequence determination and analysis, sequence comparisons, transcriptome analysis, real-time PCR expression analysis	Catharanthus roseus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
endoplasmic reticulum	an endoplasmic reticulum-anchored enzyme	Catharanthus roseus	5783	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
loganin + [reduced NADPH-hemoprotein reductase] + O2	Catharanthus roseus	-	secologanin + [oxidized NADPH-hemoprotein reductase] + 2 H2O	-	?
additional information	Catharanthus roseus	the second secologanin synthase isoform SLS2 produces both secologanin and secoxyloganin	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Catharanthus roseus	U5NDT8	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	SLS2 might be the prominent isoform of secologanin biosynthesis in the aerial parts of the plant	Catharanthus roseus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
loganin + [reduced NADPH-hemoprotein reductase] + O2	-	Catharanthus roseus	secologanin + [oxidized NADPH-hemoprotein reductase] + 2 H2O	-	?
additional information	the second secologanin synthase isoform SLS2 produces both secologanin and secoxyloganin	Catharanthus roseus	?	-	?

Synonyms

Synonyms	Comment	Organism
CYP72C	-	Catharanthus roseus
SLS	-	Catharanthus roseus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Catharanthus roseus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Catharanthus roseus

Cofactor

Cofactor	Comment	Organism	Structure
cytochrome P450	-	Catharanthus roseus
NADPH-hemoprotein reductase	-	Catharanthus roseus

General Information

General Information	Comment	Organism
metabolism	the enzyme is important in the monoterpenoid indole alkaloid (MIA) biosynthesis in Catharanthus roseus, subcellular organization of the central steps of the pathway, overview. The ring-opening reaction of loganin in the biosynthesis of secologanin is catalyzed by the fourth P450 of this pathway, secologanin synthase (SLS, CYP72A1). Both isozymes SLS1 and SLS2 not only catalyze the oxidative ring cleavage of loganin to produce secologanin but also perform the oxidation of secologanin into secoxyloganin. SLS1 and SLS2 constitute the third type of P450 from the seco-iridoid pathway performing more than one catalytic reaction. Additional enzymes might convert secoxyloganin back to secologanin, the subcellular compartmentation of secologanin biosynthesis may also limit secoxyloganin formation in planta. Secoxyloganin is an acidic compound derived from secologanin that is no longer able to be condensed with tryptamine by STR in the vacuole, due to the absence of the aldehyde function. If secoxyloganin formation occurs in vivo, the resulting depletion of the secologanin pool is deleterious for the subsequent synthesis of MIAs	Catharanthus roseus
additional information	consensus transcriptome analysis of Catharanthus roseus, detailed overview	Catharanthus roseus
physiological function	secologanin synthases (SLS) are P450 enzymes that catalyze an unusual ring-opening reaction of loganin in the biosynthesis of the monoterpenoid indole alkaloids (MIA) precursor secologanin	Catharanthus roseus