Secologanin synthase which catalyzes the oxidative cleavage of loganin into secologanin is a cytochrome P450

Yamamoto, H.; Katano, N.; Ooi, A.; Inoue, K.; Phytochemistry 53, 7-12 (2000)

Data extracted from this reference:

Inhibitors

Commentary

Organism

Structure

Ancymidol

complete inhibition

Lonicera japonica

carbon monoxide

inhibition by CO and O2 in the ratio 9 to 1 in the dark, can be reversed by illumination with white light

Lonicera japonica

cytochrome c

partial inhibition

Lonicera japonica

Metyrapone

partial inhibition

Lonicera japonica

miconazole

partial inhibition

Lonicera japonica

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

loganin + [reduced NADPH-hemoprotein reductase] + O2

Lonicera japonica

-

secologanin + [oxidized NADPH-hemoprotein reductase] + H2O

-

Lonicera japonica

?

Reaction

Commentary

Organism

Reaction ID

loganin + [reduced NADPH-hemoprotein reductase] + O2 = secologanin + [oxidized NADPH-hemoprotein reductase] + 2 H2O

enzyme belongs to the group of cytochrome P450 monooxygenases

Lonicera japonica

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

loganin + [reduced NADPH-hemoprotein reductase] + O2

-

437785

Lonicera japonica

secologanin + [oxidized NADPH-hemoprotein reductase] + H2O

-

437785

Lonicera japonica

?

loganin + [reduced NADPH-hemoprotein reductase] + O2

requires molecular oxygen for oxidative cleavage of the carbon-carbon bond

437785

Lonicera japonica

secologanin + [oxidized NADPH-hemoprotein reductase] + H2O

-

437785

Lonicera japonica

?

additional information

no substrates: loganic acid, 10-hydroxyloganin, 7-epiloganin, 7-dehydrologanin, 7-dehydrologanic acid, 8-epiloganin, verbenalin

437785

Lonicera japonica

?

-

-

-

?

Inhibitors

Commentary

Organism

Structure

Ancymidol

complete inhibition

Lonicera japonica

carbon monoxide

inhibition by CO and O2 in the ratio 9 to 1 in the dark, can be reversed by illumination with white light

Lonicera japonica

cytochrome c

partial inhibition

Lonicera japonica

Metyrapone

partial inhibition

Lonicera japonica

miconazole

partial inhibition

Lonicera japonica

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

loganin + [reduced NADPH-hemoprotein reductase] + O2

Lonicera japonica

-

secologanin + [oxidized NADPH-hemoprotein reductase] + H2O

-

Lonicera japonica

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

loganin + [reduced NADPH-hemoprotein reductase] + O2

-

437785

Lonicera japonica

secologanin + [oxidized NADPH-hemoprotein reductase] + H2O

-

437785

Lonicera japonica

?

loganin + [reduced NADPH-hemoprotein reductase] + O2

requires molecular oxygen for oxidative cleavage of the carbon-carbon bond

437785

Lonicera japonica

secologanin + [oxidized NADPH-hemoprotein reductase] + H2O

-

437785

Lonicera japonica

?

additional information

no substrates: loganic acid, 10-hydroxyloganin, 7-epiloganin, 7-dehydrologanin, 7-dehydrologanic acid, 8-epiloganin, verbenalin

437785

Lonicera japonica

?

-

-

-

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

744599

Duge de Bernonville

Characterization of a second ...

Catharanthus roseus

BMC Genomics

16

619

2015

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724967

Guirimand

The subcellular organization o ...

Catharanthus roseus

FEBS J.

278

749-763

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670558

Murata

localization of tabersonine 16 ...

Catharanthus roseus

Plant J.

44

581-594

2005

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437785

Yamamoto

Secologanin synthase which cat ...

Lonicera japonica

Phytochemistry

53

7-12

2000

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5

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437786

Irmler

Indole alkaloid biosynthesis i ...

Catharanthus roseus

Plant J.

24

797-804

2000

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437784

Yamamoto

-

Transformation of loganin and ...

Lonicera japonica

Phytochemistry

50

417-422

1999

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