Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae EH1315, 7.9% oleic acid and 29% linoleic acid in yeast expressing DELTA12 desaturase compared with 37% oleic acid and no detectable linoleic acid in control yeast, fatty acid composition analyzed by gas-liquid chromatography | Coprinopsis cinerea |
gene Cop-odeA, DNA and amino acid sequence determination and analysis, functional expression in Saccharomyces cerevisiae strain EH1315 | Coprinopsis cinerea |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a recombinant Saccharomyces cerevisiae strain EH1315 expressing gene Cop-odeA accumulates four additional fatty acids identified as 9,12-hexadecadienoic acid, 9,12,15-hexadecatrienoic acid, linoleic acid, and alinolenic acid, which comprised 8.8%, 1.0%, 29.0%, and 0.6% of the total fatty acids, respectively, overview | Coprinopsis cinerea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
oleic acid + ? | Coprinopsis cinerea | - |
linoleic acid + ? | - |
? | |
oleic acid + ? | Coprinopsis cinerea TD#822-2 | - |
linoleic acid + ? | - |
? | |
oleic acid + ? | Coprinopsis cinerea TD822-2 | - |
linoleic acid + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Coprinopsis cinerea | A2A1C4 | TD822-2 | - |
Coprinopsis cinerea | A2A1C4 | gene Cop-odeA | - |
Coprinopsis cinerea TD#822-2 | A2A1C4 | gene Cop-odeA | - |
Coprinopsis cinerea TD822-2 | A2A1C4 | TD822-2 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is a bifunctional fatty acid desaturase with both high DELTA12 desaturase activity and unusual DELTA15 desaturase activities | Coprinopsis cinerea | ? | - |
? | |
additional information | the enzyme is a bifunctional fatty acid desaturase with both high DELTA12 desaturase activity and unusual DELTA15 desaturase activities | Coprinopsis cinerea TD#822-2 | ? | - |
? | |
additional information | the enzyme is a bifunctional fatty acid desaturase with both high DELTA12 desaturase activity and unusual DELTA15 desaturase activities | Coprinopsis cinerea TD822-2 | ? | - |
? | |
oleic acid + ? | - |
Coprinopsis cinerea | linoleic acid + ? | - |
? | |
oleic acid + ? | - |
Coprinopsis cinerea TD#822-2 | linoleic acid + ? | - |
? | |
oleic acid + ? | - |
Coprinopsis cinerea TD822-2 | linoleic acid + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DELTA12DS | bifunctional enzyme with strong DELTA12 and low DELTA15 desaturase activities | Coprinopsis cinerea |