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Literature summary for 1.14.19.59 extracted from

  • Schnepel, C.; Minges, H.; Frese, M.; Sewald, N.
    A high-throughput fluorescence assay to determine the activity of tryptophan halogenases (2016), Angew. Chem. Int. Ed. Engl., 55, 14159-14163 .
    View publication on PubMed

Application

Application Comment Organism
analysis development of a quantitative halogenase assay based on a Suzuki-Miyaura cross-coupling towards the formation of a fluorescent aryltryptophan and optimization for application in crude Escherichia coli lysate Streptomyces albogriseolus

Protein Variants

Protein Variants Comment Organism
K374R thermostable tryptophan 6-halogenase mutant, increase in melting temperature by 10 degrees Streptomyces albogriseolus

Organism

Organism UniProt Comment Textmining
Streptomyces albogriseolus A1E280
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tryptophan + FADH2 + bromide + O2 + H+
-
Streptomyces albogriseolus 6-bromo-L-tryptophan + FAD + 2 H2O
-
?

Synonyms

Synonyms Comment Organism
Thal
-
Streptomyces albogriseolus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45
-
wild-type, inactive above Streptomyces albogriseolus
49
-
mutant K374R, 99% of maximum activity Streptomyces albogriseolus
56
-
mutant K374R, 71% of maximum activity Streptomyces albogriseolus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
47
-
melting temperature, wild-type Streptomyces albogriseolus
57
-
melting temperature, mutant K374R Streptomyces albogriseolus