Application | Comment | Organism |
---|---|---|
synthesis | regeneration of FADH2 using flavin dehydrogenase plus formiate dehydrogenase from Candida boidiniiat 0.28 U/ml leads to about 60% increase in yield | Streptomyces rugosporus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptomyces rugosporus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no substrate inhibition by tryptophan up to 2 mM | Streptomyces rugosporus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.071 | - |
L-tryptophan | pH 7.2, 30°C | Streptomyces rugosporus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces rugosporus | A4D0H5 | - |
- |
Streptomyces rugosporus LL-42D005 | A4D0H5 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Streptomyces rugosporus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + FADH2 + chloride + O2 + H+ | - |
Streptomyces rugosporus | 5-chloro-L-tryptophan + FAD + 2 H2O | - |
? | |
L-tryptophan + FADH2 + chloride + O2 + H+ | - |
Streptomyces rugosporus LL-42D005 | 5-chloro-L-tryptophan + FAD + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
pyrH | - |
Streptomyces rugosporus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
- |
half-life 1848 min | Streptomyces rugosporus |
22 | - |
half-life 517 min | Streptomyces rugosporus |
30 | - |
half-life 43 min | Streptomyces rugosporus |