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Literature summary for 1.14.19.58 extracted from

  • Muffler, K.; Kuetchou Ngnigha, A.; Ulber, R.
    Bestimmung kinetischer Parameter der FADH2-abhängigen Tryptophan-5-Halogenase aus Streptomyces rugosporus (2010), Chem.-Ing.-Tech., 82, 121-127 .
No PubMed abstract available
Search for more literature for 1.14.19.58

Application

Application Comment Organism
synthesis regeneration of FADH2 using flavin dehydrogenase plus formiate dehydrogenase from Candida boidiniiat 0.28 U/ml leads to about 60% increase in yield Streptomyces rugosporus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces rugosporus

Inhibitors

Inhibitors Comment Organism Structure
additional information no substrate inhibition by tryptophan up to 2 mM Streptomyces rugosporus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.071
-
 L-tryptophan pH 7.2, 30°C Streptomyces rugosporus

Organism

Organism UniProt Comment Textmining
Streptomyces rugosporus A4D0H5
-
-
Streptomyces rugosporus LL-42D005 A4D0H5
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Streptomyces rugosporus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tryptophan + FADH2 + chloride + O2 + H+
-
 Streptomyces rugosporus 5-chloro-L-tryptophan + FAD + 2 H2O
-
 ?
L-tryptophan + FADH2 + chloride + O2 + H+
-
 Streptomyces rugosporus LL-42D005 5-chloro-L-tryptophan + FAD + 2 H2O
-
 ?

Synonyms

Synonyms Comment Organism
pyrH
-
 Streptomyces rugosporus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
-
 half-life 1848 min Streptomyces rugosporus
22
-
 half-life 517 min Streptomyces rugosporus
30
-
 half-life 43 min Streptomyces rugosporus