Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.19.58 extracted from

  • Zehner, S.; Kotzsch, A.; Bister, B.; Suessmuth, R.D.; Mendez, C.; Salas, J.A.; van Pee, K.H.
    A regioselective tryptophan 5-halogenase is involved in pyrroindomycin biosynthesis in Streptomyces rugosporus LL-42D005 (2005), Chem. Biol., 12, 445-452.
    View publication on PubMed
Search for more literature for 1.14.19.58

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Pseudomonas fluorescens BL915 DELTAORF1 Streptomyces rugosporus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 L-tryptophan pH 7.2, 30°C Streptomyces rugosporus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tryptophan + FADH2 + Cl- + O2 + H+ Streptomyces rugosporus the enzyme is involved in the biosynthesis of the antibiotic pyrroindomycin B that contains an indole ring chlorinated in the 5 position 5-chloro-L-tryptophan + FAD + 2 H2O
-
 ?
L-tryptophan + FADH2 + Cl- + O2 + H+ Streptomyces rugosporus LL-42D005 the enzyme is involved in the biosynthesis of the antibiotic pyrroindomycin B that contains an indole ring chlorinated in the 5 position 5-chloro-L-tryptophan + FAD + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces rugosporus A4D0H5
-
-
Streptomyces rugosporus LL-42D005 A4D0H5
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces rugosporus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tryptophan + FADH2 + Br- + O2 + H+ brominating activity is about 75% of the chlorinating activity Streptomyces rugosporus 5-bromo-L-tryptophan + FAD + 2 H2O
-
 ?
L-tryptophan + FADH2 + Br- + O2 + H+ brominating activity is about 75% of the chlorinating activity Streptomyces rugosporus LL-42D005 5-bromo-L-tryptophan + FAD + 2 H2O
-
 ?
L-tryptophan + FADH2 + Cl- + O2 + H+
-
 Streptomyces rugosporus 5-chloro-L-tryptophan + FAD + 2 H2O
-
 ?
L-tryptophan + FADH2 + Cl- + O2 + H+ the enzyme is involved in the biosynthesis of the antibiotic pyrroindomycin B that contains an indole ring chlorinated in the 5 position Streptomyces rugosporus 5-chloro-L-tryptophan + FAD + 2 H2O
-
 ?
L-tryptophan + FADH2 + Cl- + O2 + H+
-
 Streptomyces rugosporus LL-42D005 5-chloro-L-tryptophan + FAD + 2 H2O
-
 ?
L-tryptophan + FADH2 + Cl- + O2 + H+ the enzyme is involved in the biosynthesis of the antibiotic pyrroindomycin B that contains an indole ring chlorinated in the 5 position Streptomyces rugosporus LL-42D005 5-chloro-L-tryptophan + FAD + 2 H2O
-
 ?

Synonyms

Synonyms Comment Organism
pyrH
-
 Streptomyces rugosporus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Streptomyces rugosporus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00813
-
 L-tryptophan pH 7.2, 30°C Streptomyces rugosporus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Streptomyces rugosporus

General Information

General Information Comment Organism
malfunction a gene disruption mutant in the tryptophan 5-halogenase gene no longer produces the pyrroindomycin B, but still produces pyrroindomycin A, the nonhalogenated derivative Streptomyces rugosporus
physiological function the enzyme is involved in the biosynthesis of the antibiotic pyrroindomycin B that contains an indole ring chlorinated in the 5 position Streptomyces rugosporus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0542
-
 L-tryptophan pH 7.2, 30°C Streptomyces rugosporus