Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | Q4KCZ0 | - |
- |
Pseudomonas fluorescens ATCC BAA-477 | Q4KCZ0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1H-pyrrole-2-carbonyl-[PltL peptidyl-carrier protein] + 2 FADH2 + 2 bromide + 2 O2 | - |
Pseudomonas fluorescens | 4,5-dibromo-1H-pyrrole-2-carbonyl-[PltL peptidyl-carrier protein] + 2 FAD + 4 H2O | - |
? | |
1H-pyrrole-2-carbonyl-[PltL peptidyl-carrier protein] + 2 FADH2 + 2 bromide + 2 O2 | - |
Pseudomonas fluorescens ATCC BAA-477 | 4,5-dibromo-1H-pyrrole-2-carbonyl-[PltL peptidyl-carrier protein] + 2 FAD + 4 H2O | - |
? | |
1H-pyrrole-2-carbonyl-[PltL peptidyl-carrier protein] + 2 FADH2 + 2 chloride + 2 O2 | - |
Pseudomonas fluorescens | 4,5-dichloro-1H-pyrrole-2-carbonyl-[PltL peptidyl-carrier protein] + 2 FAD + 4 H2O | - |
? | |
1H-pyrrole-2-carbonyl-[PltL peptidyl-carrier protein] + 2 FADH2 + 2 chloride + 2 O2 | - |
Pseudomonas fluorescens ATCC BAA-477 | 4,5-dichloro-1H-pyrrole-2-carbonyl-[PltL peptidyl-carrier protein] + 2 FAD + 4 H2O | - |
? | |
1H-pyrrole-2-carbonyl-[PltL peptidyl-carrier protein] + FADH2 + chloride + O2 | - |
Pseudomonas fluorescens | 5-chloro-1H-pyrrole-2-carbonyl-[PltL peptidyl-carrier protein] + FAD + 2 H2O | - |
? | |
1H-pyrrole-2-carbonyl-[PltL peptidyl-carrier protein] + FADH2 + chloride + O2 | - |
Pseudomonas fluorescens ATCC BAA-477 | 5-chloro-1H-pyrrole-2-carbonyl-[PltL peptidyl-carrier protein] + FAD + 2 H2O | - |
? | |
5-chloro-1H-pyrrole-2-carbonyl-[PltL peptidyl-carrier protein] + FADH2 + chloride + O2 | - |
Pseudomonas fluorescens | 4,5-dichloro-1H-pyrrole-2-carbonyl-[PltL peptidyl-carrier protein] + FAD + H2O | - |
? | |
5-chloro-1H-pyrrole-2-carbonyl-[PltL peptidyl-carrier protein] + FADH2 + chloride + O2 | - |
Pseudomonas fluorescens ATCC BAA-477 | 4,5-dichloro-1H-pyrrole-2-carbonyl-[PltL peptidyl-carrier protein] + FAD + H2O | - |
? | |
additional information | halogenase PltA catalyzes chlorination at both positions of the ring, generating the 5-chloropyrrolyl-S-PltL intermediate and then the 4,5-dichloropyrrolyl-S-PltL product. The regiospecific chlorination occurs at position 5 and then position 4 of the heteroaromatic ring. The mechanism may involve formation of a FAD-4a-OCl intermediate for capture by the electron-rich C4 and C5 of the heteroaromatic pyrrole moiety | Pseudomonas fluorescens | ? | - |
? | |
additional information | halogenase PltA catalyzes chlorination at both positions of the ring, generating the 5-chloropyrrolyl-S-PltL intermediate and then the 4,5-dichloropyrrolyl-S-PltL product. The regiospecific chlorination occurs at position 5 and then position 4 of the heteroaromatic ring. The mechanism may involve formation of a FAD-4a-OCl intermediate for capture by the electron-rich C4 and C5 of the heteroaromatic pyrrole moiety | Pseudomonas fluorescens ATCC BAA-477 | ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | purified PltA contains tightly bound FAD | Pseudomonas fluorescens |
General Information | Comment | Organism |
---|---|---|
physiological function | the antifungal natural product pyoluteorin contains a 4,5-dichloropyrrole moiety. Dichlorination in the heteroaromatic ring occurs after proline is tethered by thioester linkage to the carrier protein PltL and enzymatically desaturated to the pyrrolyl-S-PltL. The FADH2-dependent halogenase PltA catalyzes chlorination at both positions of the ring, generating the 5-chloropyrrolyl-S-PltL intermediate and then the 4,5-dichloropyrrolyl-S-PltL product. PltA activity strictly depends on a heterologous flavin reductase that uses NAD(P)H to produce FADH2 | Pseudomonas fluorescens |