BRENDA - Enzyme Database
show all sequences of 1.14.19.54

Plant science. Morphinan biosynthesis in opium poppy requires a P450-oxidoreductase fusion protein

Winzer, T.; Kern, M.; King, A.J.; Larson, T.R.; Teodor, R.I.; Donninger, S.L.; Li, Y.; Dowle, A.A.; Cartwright, J.; Bates, R.; Ashford, D.; Thomas, J.; Walker, C.; Bowser, T.A.; Graham, I.A.; Science 349, 309-312 (2015)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.013
-
(S)-reticuline
Cyp82Y2 module, pH not specified in the publication, temperature not specified in the publication
Papaver somniferum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Papaver somniferum
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Papaver somniferum
P0DKI7
bifunctional protein, the P450 module is responsible for the conversion of (S)-reticuline to 1,2-dehydroreticuline, whereas the oxidoreductase module converts 1,2-dehydroreticuline to (R)-reticuline rather than functioning as a P450 redox partner
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Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-reticuline + [reduced NADPH-hemoprotein reductase] + O2
-
743843
Papaver somniferum
1,2-dehydroreticuline + [oxidized NADPH-hemoprotein reductase] + 2 H2O
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-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Papaver somniferum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Papaver somniferum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.013
-
(S)-reticuline
Cyp82Y2 module, pH not specified in the publication, temperature not specified in the publication
Papaver somniferum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Papaver somniferum
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-reticuline + [reduced NADPH-hemoprotein reductase] + O2
-
743843
Papaver somniferum
1,2-dehydroreticuline + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
Other publictions for EC 1.14.19.54
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743332
Farrow
Stereochemical inversion of ( ...
Papaver rhoeas, Papaver somniferum
Nat. Chem. Biol.
11
728-732
2015
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2
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2
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8
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2
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2
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8
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2
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743843
Winzer
Plant science. Morphinan bios ...
Papaver somniferum
Science
349
309-312
2015
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1
1
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1
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1
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1
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746006
Hirata
1,2-Dehydroreticuline synthas ...
Papaver somniferum
Phytochemistry
65
1039-1046
2004
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1
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1
1
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2
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1
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3
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2
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1
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