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Literature summary for 1.14.19.49 extracted from

  • Zhu, T.; Cheng, X.; Liu, Y.; Deng, Z.; You, D.
    Deciphering and engineering of the final step halogenase for improved chlortetracycline biosynthesis in industrial Streptomyces aureofaciens (2013), Metab. Eng., 19, 69-78.
    View publication on PubMed
Search for more literature for 1.14.19.49

Application

Application Comment Organism
synthesis a strain lacking CtcP activity accumulates tetracycline with a yield of 18.9 g/l. Overexpressionof ctcP in Streptomyces aureofaciens leads to CTC production to a final titer of 25.9g/l Kitasatospora aureofaciens

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Kitasatospora aureofaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.056
-
 (4S)-tetracycline pH 7.4, 30°C Kitasatospora aureofaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66300
-
-
 Kitasatospora aureofaciens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(4S)-tetracycline + FADH2 + chloride + O2 + H+ Kitasatospora aureofaciens enzyme is specific for (4S)-tetracycline 7-chloro-(4S)-tetracycline + FAD + 2 H2O
-
 ?
(4S)-tetracycline + FADH2 + chloride + O2 + H+ Kitasatospora aureofaciens F3 enzyme is specific for (4S)-tetracycline 7-chloro-(4S)-tetracycline + FAD + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Kitasatospora aureofaciens S4S3E1
-
-
Kitasatospora aureofaciens F3 S4S3E1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(4S)-tetracycline + FADH2 + chloride + O2 + H+ enzyme is specific for (4S)-tetracycline Kitasatospora aureofaciens 7-chloro-(4S)-tetracycline + FAD + 2 H2O
-
 ?
(4S)-tetracycline + FADH2 + chloride + O2 + H+ enzyme is specific for (4S)-tetracycline Kitasatospora aureofaciens F3 7-chloro-(4S)-tetracycline + FAD + 2 H2O
-
 ?
tetracycline + FADH2 + chloride + O2 + H+
-
 Kitasatospora aureofaciens 7-chlorotetracycline + FAD + 2 H2O
-
 ?
tetracycline + FADH2 + chloride + O2 + H+
-
 Kitasatospora aureofaciens F3 7-chlorotetracycline + FAD + 2 H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 66300, SDS-PAGE of recombinant protein with His-tag Kitasatospora aureofaciens

Synonyms

Synonyms Comment Organism
ctcP
-
 Kitasatospora aureofaciens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0085
-
 (4S)-tetracycline pH 7.4, 30°C Kitasatospora aureofaciens

Cofactor

Cofactor Comment Organism Structure
FADH2
-
 Kitasatospora aureofaciens

General Information

General Information Comment Organism
physiological function chlorination by FADH2-dependent halogenase CtcP is the last inefficient step during chlortetracycline biosynthesis. A strain lacking CtcP activity accumulates tetracycline and is deficient in production of chlortetracycline Kitasatospora aureofaciens