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Literature summary for 1.14.19.45 extracted from

  • Rodriguez-Rodriguez, M.; Salas, J.; Venegas-Caleron, M.; Garces, R.; Martinez-Force, E.
    Molecular cloning and characterization of the genes encoding a microsomal oleate DELTA12 desaturase (CsFAD2) and linoleate DELTA15 desaturase (CsFAD3) from Camelina sativa (2016), Ind. Crops Prod., 89, 405-415 .
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Cloned(Commentary)

Cloned (Comment) Organism
gene CsFAD2, variant FAD2-1, genetic structure, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, subcloning in Escherichia coli strain XL1-Blue, and functional recombinant expression in Saccharomyces cerevisiae strain W303-1A. The recombinant CsFAD2 shows conversion of 18:1DELTA9 to 18:2DELTA9,12, slightly better at 30°C than at 22°C, and it is also able to desaturate palmitoleic acid (16:1DELTA9) to hexadecadienoic acid (16:2DELTA9,12). Fatty acid composition of Saccharomyces cerevisiae cells overexpressing CsFAD2 microsomal desaturase and grown at two different temperatures, overview Camelina sativa
gene CsFAD2, variant FAD2-2, genetic structure, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, subcloning in Escherichia coli strain XL1-Blue, and functional recombinant expression in Saccharomyces cerevisiae strain W303-1A. The recombinant CsFAD2 shows conversion of 18:1DELTA9 to 18:2DELTA9,12, slightly better at 30°C than at 22°C, and it is also able to desaturate palmitoleic acid (16:1DELTA9) to hexadecadienoic acid (16:2DELTA9,12). Fatty acid composition of Saccharomyces cerevisiae cells overexpressing CsFAD2 microsomal desaturase and grown at two different temperatures, overview Camelina sativa
gene CsFAD2, variant FAD2-3, genetic structure, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, subcloning in Escherichia coli strain XL1-Blue, and functional recombinant expression in Saccharomyces cerevisiae strain W303-1A . The recombinant CsFAD2 shows conversion of 18:1DELTA9 to 18:2DELTA9,12, slightly better at 30°C than at 22°C, and it is also able to desaturate palmitoleic acid (16:1DELTA9) to hexadecadienoic acid (16:2DELTA9,12). Fatty acid composition of Saccharomyces cerevisiae cells overexpressing CsFAD2 microsomal desaturase and grown at two different temperatures, overview Camelina sativa

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Camelina sativa 5783
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membrane integral membrane enzyme Camelina sativa 16020
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microsome
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 Camelina sativa
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ two Fe2+ ions bound in the His box Camelina sativa

Organism

Organism UniProt Comment Textmining
Camelina sativa E7CCB4 FAD2-2
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Camelina sativa E7CCB6 FAD2-1
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Camelina sativa M4HN37 FAD2-3
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Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
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 Camelina sativa
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hypocotyl
-
 Camelina sativa
-
leaf
-
 Camelina sativa
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root
-
 Camelina sativa
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seed high expression level, particularly from 6 to 12 DAF and from 18 to 24 DAF,corresponding with the phases of seed expansion and oil accumula-tion respectively Camelina sativa
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seed high expression level, particularly from 6 to 12 days after flowering and from 18 to 24 days after flowering , corresponding with the phases of seed expansion and oil accumulation respectively Camelina sativa
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Subunits

Subunits Comment Organism
? x * 44100, about, sequence calculation Camelina sativa

Synonyms

Synonyms Comment Organism
CsFAD2
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 Camelina sativa
FAD2
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 Camelina sativa
FAD2-1
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 Camelina sativa
FAD2-2
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 Camelina sativa
FAD2-3
-
 Camelina sativa
oleate DELTA12desaturase
-
 Camelina sativa

pI Value

Organism Comment pI Value Maximum pI Value
Camelina sativa sequence calculation
-
 8
Camelina sativa sequence calculation
-
 8.1

General Information

General Information Comment Organism
evolution three different copies of the genes FAD2 and FAD3 are identified, which contain three histidine rich motifs (HXCGHX, HRXHH andHVXHH) and six highly conserved transmembrane domains. Comparing their sequences, the CsFAD2copies accommodate four conservative changes (E36D, R48H, V97A, and A177P) and two semi-conservative ones (V63I and L249M), whereas only one semi-conservative change (A327S) is detected in CsFAD3 but with two extra amino acids (H147 and G148) Camelina sativa