KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
beta-sitosterol | isoform CYP710A1, pH and temperature not specified in the publication | Arabidopsis thaliana | |
0.0037 | - |
beta-sitosterol | isoform CYP710A2, pH and temperature not specified in the publication | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ergosta-5,7,24(28)-trien-3beta-ol + NADPH + H+ + O2 | Arabidopsis thaliana | - |
ergosta-5,7,22,24(28)-tetraen-3-beta-ol + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O64697 | - |
- |
Arabidopsis thaliana | O64698 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
24-epi-campesterol + NADPH + H+ + O2 | substrate for isoform CYP710A2 but not CYP710A1 | Arabidopsis thaliana | brassicasterol + crinosterol + NADP+ + H2O | brassicasterol is the major product | ? | |
beta-sitosterol + NADPH + H+ + O2 | high affinity substrate | Arabidopsis thaliana | stigmasterol + NADP+ + H2O | - |
? | |
ergosta-5,7,24(28)-trien-3beta-ol + NADPH + H+ + O2 | - |
Arabidopsis thaliana | ergosta-5,7,22,24(28)-tetraen-3-beta-ol + NADP+ + H2O | - |
? | |
additional information | 24-epi-campesterol is no substrate for isoform CYP710A1 | Arabidopsis thaliana | ? | brassicasterol is the major product | ? |
Synonyms | Comment | Organism |
---|---|---|
CYP710A1 | isoform | Arabidopsis thaliana |
CYP710A2 | isoform | Arabidopsis thaliana |
sterol C-22 desaturase | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
beta-sitosterol | isoform CYP710A1, pH and temperature not specified in the publication | Arabidopsis thaliana | |
0.17 | - |
beta-sitosterol | isoform CYP710A2, pH and temperature not specified in the publication | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Arabidopsis thaliana |