Cloned (Comment) | Organism |
---|---|
expressed in yeast cells | Helianthus annuus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helianthus annuus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-9-fluorophytosphinganine + reduced acceptor + O2 | exclusively produces (Z)-9-fluoro-8-phytosphingenine. This result is explained by an initial attack onto the C8-HR and syn-elimination of two vicinal hydrogen atoms via an anti-conformation of the substrate. An initial attack on the C9-F is excluded and generates no product | Helianthus annuus | (Z)-9-fluoro-8-phytosphingenine + acceptor + H2O | - |
? | |
(S)-8-fluorophytosphinganine + reduced acceptor + O2 | product predominantly transformed to (Z)-8-fluoro-8-phytosphingenine along with only trace amounts of the (E)-8-fluoro-8-phytosphingenine (ratio 95:5). This ratio indicates that the fluorinated substrate strongly favors the anti-configuration at the active center of the desaturase | Helianthus annuus | (Z)-8-fluoro-8-phytosphingenine + acceptor + H2O | - |
? | |
(S)-9-fluorophytosphinganine + reduced acceptor + O2 | produces (E)-9-fluoro-8-phytosphingenine as the sole reaction product. Only the initial attack on the C9-HR in combination with the gauche-conformation of the substrate is productive | Helianthus annuus | (E)-9-fluoro-8-phytosphingenine + acceptor + H2O | - |
? | |
additional information | (R)-8-fluorophytosphinganine does not yield any desaturation product | Helianthus annuus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DELTA8-sphingolipid desaturase | - |
Helianthus annuus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Helianthus annuus |