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Literature summary for 1.14.19.3 extracted from

  • Huang, S.; Liu, R.; Niu, Y.; Hasi, A.
    Cloning and functional characterization of a fatty acid DELTA6-desaturase from Oenothera biennis: Production of alpha-linolenic acid by heterologous expression in Saccharomyces cerevisiae (2010), Russ. J. Plant Physiol., 57, 568-573.
No PubMed abstract available
Search for more literature for 1.14.19.3

Application

Application Comment Organism
synthesis Oenothera biennis is grown commercially for gamma-linolenic acid production by the fatty acid DELTA6-desaturase enzymatic reaction Oenothera biennis

Cloned(Commentary)

Cloned (Comment) Organism
gene ObD6Des, DNA and amino acid sequence determination and analysis, sequence comparisons, functional expression in Saccharomyces cerevisiae strain W303-a Oenothera biennis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-linoleoyl-CoA + AH2 + O2 Oenothera biennis
-
 stearidonoyl-CoA + A + 2 H2O
-
 ?
linoleoyl-CoA + AH2 + O2 Oenothera biennis
-
 gamma-linolenoyl-CoA + A + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Oenothera biennis E1U2Q3 gene ObD6Des
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-linoleoyl-CoA + AH2 + O2
-
 Oenothera biennis stearidonoyl-CoA + A + 2 H2O
-
 ?
linoleoyl-CoA + AH2 + O2
-
 Oenothera biennis gamma-linolenoyl-CoA + A + 2 H2O
-
 ?

Subunits

Subunits Comment Organism
More the enzyme sequence comprises the characteristics of membrane-bound desaturases, including three conserved histidine-rich motifs, hydropathy profile, and a cytochrome b5 domain at the N-terminus, overview Oenothera biennis

Synonyms

Synonyms Comment Organism
D6Des
-
 Oenothera biennis
fatty acid DELTA6-desaturase
-
 Oenothera biennis

Cofactor

Cofactor Comment Organism Structure
cytochrome b5 binds at the N-terminus of the enzyme Oenothera biennis

General Information

General Information Comment Organism
physiological function fatty acid DELTA6-desaturase is the essential enzyme for polyunsaturated fatty acids biosynthesis and is responsible for the first step by converting linoleic acid to gamma-linolenoyl-CoA, and alpha-linolenic acid to stearidonic acid Oenothera biennis