Literature summary for 1.14.19.28 extracted from

Garba, L.; Ali, M.S.; Oslan, S.N.; Rahman, R.N.
Heterologous expression of PA8FAD9 and functional characterization of a DELTA9-fatty acid desaturase from a cold-tolerant Pseudomonas sp. A8 (2016), Mol. Biotechnol., 58, 718-728 .

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Cloned(Commentary)

Cloned (Comment)	Organism
gene PA8FAD9, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli strain Transetta (DE3), ability of the enzyme to convert exogenous stearic acid to oleic acid incorporated into the recombinant Escherichia coli membrane phospholipids. GCMS analysis confirms significant changes in the pattern of palmitoleic and palmitic acid production in Escherichia coli cells expressing PA8FAD9 gene. At 20°C, the amount of palmitoleic increased from 10.5 to 21% suggesting that PA8FAD9 gene from Pseudomonas sp. A8 codes for an active DELTA9-fatty acid desaturase-like protein	Pseudomonas sp.

Cloned (Comment)

Organism

gene PA8FAD9, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli strain Transetta (DE3), ability of the enzyme to convert exogenous stearic acid to oleic acid incorporated into the recombinant Escherichia coli membrane phospholipids. GCMS analysis confirms significant changes in the pattern of palmitoleic and palmitic acid production in Escherichia coli cells expressing PA8FAD9 gene. At 20°C, the amount of palmitoleic increased from 10.5 to 21% suggesting that PA8FAD9 gene from Pseudomonas sp. A8 codes for an active DELTA9-fatty acid desaturase-like protein

Pseudomonas sp.

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Pseudomonas sp.	16020	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+	Pseudomonas sp.	-	1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O	-	?
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+	Pseudomonas sp. A8(2016b)	-	1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas sp.	A0A165DNG6	-	-
Pseudomonas sp. A8(2016b)	A0A165DNG6	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+	-	Pseudomonas sp.	1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O	-	?
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+	-	Pseudomonas sp. A8(2016b)	1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O	-	?

Subunits

Subunits	Comment	Organism
?	x * 45000, about, sequence calculation	Pseudomonas sp.

Synonyms

Synonyms	Comment	Organism
DELTA9-fatty acid desaturase	-	Pseudomonas sp.
DELTA9-fatty acid desaturase-like protein	-	Pseudomonas sp.
FAD9	-	Pseudomonas sp.
PA8FAD9	-	Pseudomonas sp.

Cofactor

Cofactor	Comment	Organism	Structure
Ferredoxin	-	Pseudomonas sp.

General Information

General Information	Comment	Organism
physiological function	the D9-fatty acid desaturase initiates the synthesis of polyunsaturated fatty acids by introducing the first double bond at the C9 position of saturated fatty acid chains. These substrates can further be elongated by other classes of desaturase enzymes	Pseudomonas sp.