Cloned (Comment) | Organism |
---|---|
gene PA8FAD9, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli strain Transetta (DE3), ability of the enzyme to convert exogenous stearic acid to oleic acid incorporated into the recombinant Escherichia coli membrane phospholipids. GCMS analysis confirms significant changes in the pattern of palmitoleic and palmitic acid production in Escherichia coli cells expressing PA8FAD9 gene. At 20°C, the amount of palmitoleic increased from 10.5 to 21% suggesting that PA8FAD9 gene from Pseudomonas sp. A8 codes for an active DELTA9-fatty acid desaturase-like protein | Pseudomonas sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Pseudomonas sp. | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | Pseudomonas sp. | - |
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | Pseudomonas sp. A8(2016b) | - |
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | A0A165DNG6 | - |
- |
Pseudomonas sp. A8(2016b) | A0A165DNG6 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | - |
Pseudomonas sp. | 1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | - |
Pseudomonas sp. A8(2016b) | 1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, about, sequence calculation | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
DELTA9-fatty acid desaturase | - |
Pseudomonas sp. |
DELTA9-fatty acid desaturase-like protein | - |
Pseudomonas sp. |
FAD9 | - |
Pseudomonas sp. |
PA8FAD9 | - |
Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | - |
Pseudomonas sp. |
General Information | Comment | Organism |
---|---|---|
physiological function | the D9-fatty acid desaturase initiates the synthesis of polyunsaturated fatty acids by introducing the first double bond at the C9 position of saturated fatty acid chains. These substrates can further be elongated by other classes of desaturase enzymes | Pseudomonas sp. |