Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli | Thunbergia alata |
Protein Variants | Comment | Organism |
---|---|---|
A181T/A188G/Y189F/S205N/L206T/G207A | the mutation yields an enzyme that functions primarily as a DELTA9-18:0-[acyl-carrier protein] desaturase. This enzyme displays only DELTA9 desaturase activity with 18:0-[acyl-carrier protein] and is nearly 4-fold more active with this substrate than with 16:0-[acyl-carrier protein]. Like mutant A181TyA200F, this enzyme retains DELTA6 desaturase activity with 16:0-[acyl-carrier protein] | Thunbergia alata |
A181T/A200F | the mutation gives rise to an enzyme that catalyzed primarily the DELTA9 desaturation of 18:0-[acyl-carrier protein], but functions as a DELTA6 desaturase with 16:0-[acyl-carrier protein] | Thunbergia alata |
A181T/A200F/S205N/L206T/G207A | replacement of specific amino acid residues in a DELTA6-palmitoyl (16:0)-[acyl-carrier protein] desaturase with their equivalents from a DELTA9-stearoyl (18:0)-[acyl-carrier protein] desaturase. The A181T/A200F/S205N/L206T/G207A mutant enzyme is functions principally as a DELTA9-18:0-[acyl-carrier protein] desaturase | Thunbergia alata |
A188G/Y189F | mutant with broadened fatty acid chain-length specificity | Thunbergia alata |
additional information | replacement of specific amino acid residues in a DELTA6-palmitoyl (16:0)-[acyl-carrier protein] desaturase with their equivalents from a DELTA9-stearoyl (18:0)-[acyl-carrier protein] desaturase, mutant enzymes are identified that have altered fatty acid chain-length specificities or that can insert double bonds into either the DELTA6 or DELTA9 positions of 16:0- and 18:0-[acyl-carrier protein] | Thunbergia alata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
palmitoyl-[acyl-carrier protein] | Km-values determined for the wild-type enzyme and mutant A188G/Y189F are in the range of 0.2 to 0.6 mM with both 16:0- and 18:0-[acyl-carrier protein], suggesting that, at least in the case of these enzymes, changes in the substrate binding properties can be discounted as an underlying cause of differences in activities | Thunbergia alata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | Thunbergia alata | - |
(Z)-hexadec-6-enoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thunbergia alata | Q41510 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thunbergia alata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | - |
Thunbergia alata | (Z)-hexadec-6-enoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DELTA6-16:0-ACP desaturase | - |
Thunbergia alata |
DELTA6-palmitoyl (16:0)-ACP desaturase | - |
Thunbergia alata |