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Literature summary for 1.14.19.21 extracted from

  • Sathapondecha, P.; Panyim, S.; Udomkit, A.
    An essential role of Rieske domain oxygenase Neverland in the molting cycle of black tiger shrimp, Penaeus monodon (2017), Comp. Biochem. Physiol. A, 213, 11-19 .
    View publication on PubMed
Search for more literature for 1.14.19.21

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amiino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative RT-PCR expression analysis Penaeus monodon

Protein Variants

Protein Variants Comment Organism
additional information construction and expression of double-stranded RNA specific to PmNvd (dsNvd) for silencing of the enzyme, effect of PmNvd knockdown on molting duration, phenotype, overview Penaeus monodon

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane the enzyme sequence contains the transmembrane motif 33WxxF59 Penaeus monodon 16020
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Metals/Ions

Metals/Ions Comment Organism Structure
Iron the enzyme structure contains a Rieske [2Fe-2S] domain motif 107AxxG209 with the highly conserved sequence C-X-H-X16-17-C-X2-H and a non-heme iron-binding motif E-X3-D-X2-H-X4-H Penaeus monodon

Organism

Organism UniProt Comment Textmining
Penaeus monodon A0A2I4PEL8
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Source Tissue

Source Tissue Comment Organism Textmining
additional information the expression of PmNvd is dominantly in the Y-organ, but changes during molting period. The PmNvd mRNA level is low in intermolt and early premolt stages, then dramatically increases in the mid premolt stage. No enzyme expression in eyestalk, brain, thoracic ganglia, nerve cord, hepatopancreas, ovary, gills, muscle, and lymphoid organ, RT-PCR expression analysis Penaeus monodon
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Y-organ exclusive expression Penaeus monodon
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesterol + O2 + NAD(P)H + H+
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 Penaeus monodon cholesta-5,7-dien-3beta-ol + NAD(P)+ + 2 H2O
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 ?

Synonyms

Synonyms Comment Organism
cholesterol 7,8-dehydrogenase
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 Penaeus monodon
PmNvd
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 Penaeus monodon
Rieske domain oxygenase Neverland
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 Penaeus monodon

Cofactor

Cofactor Comment Organism Structure
[2Fe-2S]-center the enzyme structure contains a Rieske [2Fe-2S] domain motif 107AxxG209 with the highly conserved sequence C-X-H-X16-17-C-X2-H and a non-heme iron-binding motif E-X3-D-X2-H-X4-H Penaeus monodon

General Information

General Information Comment Organism
malfunction silencing of PmNvd transcript in shrimp by specific double-stranded RNA (dsNvd) leads to prolonged molt duration with abnormal molting progression, i.e. the molting process got stuck at early premolt stage. In addition, the 20-hydroxyecdysone titer in hemolymph of dsNvd-injected shrimps is significantly reduced compared with that in NaCl-injected shrimps. Phenotype, overview Penaeus monodon
metabolism the cholesterol 7,8-dehydrogenase catalyzes the first step of the ecdysteroid biosynthesis converting cholesterol to 7-dehydrocholesterol Penaeus monodon
physiological function function of the enzyme in the molting process and molt regulation, crucial role of PmNvd in molt progression, particularly during the initiation of premolt phase via the regulation of ecdysteroid production Penaeus monodon