Literature summary for 1.14.19.2 extracted from

Zhao, N.; Zhang, Y.; Li, Q.; Li, R.; Xia, X.; Qin, X.; Guo, H.
Identification and expression of a stearoyl-ACP desaturase gene responsible for oleic acid accumulation in Xanthoceras sorbifolia seeds (2015), Plant Physiol. Biochem., 87, 9-16 .

Search for more literature for 1.14.19.2

Cloned(Commentary)

Cloned (Comment)	Organism
gene sad, cloning from embryo, DNA and amino acid sequence determination and analysis, quantitative and semi-quantitative real-time RT-PCR enzyme expression analysis, recombinant XsSAD expression in Escherichia coli cells resulting in increased 18:1D9 level, and confirming the biological activity of the enzyme encoded by XsSAD. Recombinant XsSAD expression in and partial complementation of Arabidopsis thaliana ssi2 mutant, via Agrobacterium strain GV3101 transformation method,control of 35S promoter partially restors the morphological phenotype and effectively increases the 18:1D9 level. The levels of other unsaturated fatty acids synthesized with 18:1D9 as the substrate also increase to some degree	Xanthoceras sorbifolium

Cloned (Comment)

Organism

gene sad, cloning from embryo, DNA and amino acid sequence determination and analysis, quantitative and semi-quantitative real-time RT-PCR enzyme expression analysis, recombinant XsSAD expression in Escherichia coli cells resulting in increased 18:1D9 level, and confirming the biological activity of the enzyme encoded by XsSAD. Recombinant XsSAD expression in and partial complementation of Arabidopsis thaliana ssi2 mutant, via Agrobacterium strain GV3101 transformation method,control of 35S promoter partially restors the morphological phenotype and effectively increases the 18:1D9 level. The levels of other unsaturated fatty acids synthesized with 18:1D9 as the substrate also increase to some degree

Xanthoceras sorbifolium

Protein Variants

Protein Variants	Comment	Organism
additional information	XsSAD expression in the Arabidopsis thaliana ssi2 mutant partially complements the morphological phenotype of Arabidopsis thaliana ssi2 plants. Fatty acid composition of wild-type and mutant plants, overview	Xanthoceras sorbifolium

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast stroma	-	Xanthoceras sorbifolium	9570	-
soluble	-	Xanthoceras sorbifolium	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	ferredoxin-[iron-sulfur] cluster	Xanthoceras sorbifolium

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
stearoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+	Xanthoceras sorbifolium	-	oleoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Xanthoceras sorbifolium	A0A0K0MC84	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
embryo	high enzyme content	Xanthoceras sorbifolium	-
leaf	low enzyme content	Xanthoceras sorbifolium	-
additional information	XsSAD Xanthoceras sorbifolia has a much higher expression in embryos than in leaves and petals. XsSAD expression also correlated well with the oleic acid, unsaturated fatty acid, and total fatty acid levels in developing embryos	Xanthoceras sorbifolium	-
petal	low enzyme content	Xanthoceras sorbifolium	-
seed	-	Xanthoceras sorbifolium	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
stearoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+	-	Xanthoceras sorbifolium	oleoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O	-	?

Subunits

Subunits	Comment	Organism
?	x * 45510, sequence calculation	Xanthoceras sorbifolium

Synonyms

Synonyms	Comment	Organism
SAD	-	Xanthoceras sorbifolium
stearoyl-ACP desaturase	-	Xanthoceras sorbifolium
XsSAD	-	Xanthoceras sorbifolium

Cofactor

Cofactor	Comment	Organism	Structure
Ferredoxin	-	Xanthoceras sorbifolium

pI Value

Organism	Comment	pI Value Maximum	pI Value
Xanthoceras sorbifolium	sequence calculation	-	6.24

General Information

General Information	Comment	Organism
malfunction	fatty acid composition of wild-type and mutant plants, overview	Xanthoceras sorbifolium
physiological function	stearoyl-ACP desaturase is a plastid-localized soluble desaturase that catalyzes the conversion of stearic acid (18:0) to oleic acid, which plays a key role in determining the ratio of saturated to unsaturated fatty acids	Xanthoceras sorbifolium