Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GT11 | - |
Homo sapiens | |
additional information | DELTA6-monoenoic ceramide inhibit Des1 | Homo sapiens | |
N-((2S,3R,6E)-1,3-dihydroxyoctadec-6-en-2-yl)octanamide | substrate inhibition, non-competitive type of inhibition | Homo sapiens | |
N-((2S,3R,6Z)-1,3-dihydroxyoctadec-6-en-2-yl)octanamide | substrate inhibition | Homo sapiens | |
XM462 | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a dihydroceramide + 2 ferrocytochrome b5 + O2 + 2 H+ | Homo sapiens | - |
a (4E)-sphing-4-enine ceramide + 2 ferricytochrome b5 + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HGC-27 cell | - |
Homo sapiens | - |
T-98G cell | - |
Homo sapiens | - |
U-87MG cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a dihydroceramide + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Homo sapiens | a (4E)-sphing-4-enine ceramide + 2 ferricytochrome b5 + 2 H2O | - |
? | |
additional information | configurational preference of dihydroceramide desaturase-1 towards DELTA6-unsaturated substrates. Establishment of an assay for Des1 activity based on the use of an immobilized DELTA6-monoene as a substrate for further click reaction of the resulting diene with a labelable dienophile, chemical synthesis of DELTA6 and DELTA4,6 sphingoid bases, substrate specificity, overview | Homo sapiens | ? | - |
? | |
N-((2S,3R,6E)-1,3-dihydroxyoctadec-6-en-2-yl)-6-((7-nitrobenzo[c][1,2,5]oxadiazol-4-yl)amino)hexanamide + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Homo sapiens | N-((2S,3R,4E,6E)-1,3-dihydroxyoctadeca-4,6-dien-2-yl)-6-((7-nitrobenzo[c][1,2,5]oxadiazol-4-yl)amino)hexanamide + 2 ferricytochrome b5 + 2 H2O | - |
? | |
N-((2S,3R,6E)-1,3-dihydroxyoctadec-6-en-2-yl)octanamide + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Homo sapiens | N-((2S,3R,4E,6E)-1,3-dihydroxyoctadeca-4,6-dien-2-yl)octanamide + 2 ferricytochrome b5 + 2 H2O | - |
? | |
N-((2S,3R,6Z)-1,3-dihydroxyoctadec-6-en-2-yl)-6-((7-nitrobenzo[c][1,2,5]oxadiazol-4-yl)amino)hexanamide + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Homo sapiens | N-((2S,3R,4E,6Z)-1,3-dihydroxyoctadeca-4,6-dien-2-yl)-6-((7-nitrobenzo[c][1,2,5]oxadiazol-4-yl)amino)hexanamide + 2 ferricytochrome b5 + 2 H2O | - |
? | |
N-((2S,3R,6Z)-1,3-dihydroxyoctadec-6-en-2-yl)octanamide + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Homo sapiens | N-((2S,3R,4E,6Z)-1,3-dihydroxyoctadeca-4,6-dien-2-yl)octanamide + 2 ferricytochrome b5 + 2 H2O | - |
? | |
N-octanoylsphinganine + ferrocytochrome b5 + O2 + 2 H+ | - |
Homo sapiens | N-octanoylsphingosine + ferricytochrome b5 + 2 H2O | - |
? | |
N-[6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl]-D-erythro-sphinganine + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DES1 | - |
Homo sapiens |
dihydroceramide desaturase-1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome b5 | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000022 | - |
N-((2S,3R,6Z)-1,3-dihydroxyoctadec-6-en-2-yl)octanamide | pH 7.4, 37°C | Homo sapiens | |
0.0001114 | - |
N-((2S,3R,6E)-1,3-dihydroxyoctadec-6-en-2-yl)octanamide | pH 7.4, 37°C | Homo sapiens | |
0.002 | - |
XM462 | pH 7.4, 37°C | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000052 | - |
pH 7.4, 37°C | Homo sapiens | N-((2S,3R,6Z)-1,3-dihydroxyoctadec-6-en-2-yl)octanamide |
General Information | Comment | Organism |
---|---|---|
metabolism | in the de novo synthesis, ceramide synthases catalyze the N-acylation of sphinganine to dihydroceramide, which is finally converted to ceramide by introduction of an (E)-4 double bond. The last step is catalyzed by dihydroceramide desaturase 1 (Des1), the enzyme that regulates the balance between dihydroceramide and ceramide | Homo sapiens |
physiological function | dihydroceramide desaturase 1 (Des1) catalyzes the last step of ceramide synthesis de novo, thus regulating the physiologically relevant balance between dihydrosphingolipids and sphingolipids | Homo sapiens |